Quan Changyun, Zhang Yue, Liu Yuanyuan, Wen Liping, Yang Haixia, Huang Xueqin, Yang Minghui, Xu Binjie
Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Cofoe Medical Technology Co., Ltd., No. 816 Zhenghua Road, Changsha 410021, China.
Biosensors (Basel). 2024 Dec 27;15(1):9. doi: 10.3390/bios15010009.
Electrochemical glucose sensor holds significant promise for the monitoring of blood glucose levels in diabetic patients. In this study, we proposed a novel electrochemical glucose sensor based on 1,10-Phenanthroline-5,6-dione (PD)/Ru(III) as a dual redox mediator. The synergistic effect of PD and Ru(III) was utilized to efficiently facilitate the electron transfer between the enzyme-active center and the electrode. Then, a commercial disposable electrochemical glucose sensor was constructed based on screen-printing electrodes. Experimental results indicated the synergy between PD and Ru(III) provided a promising electron transfer environment for a glucose dehydrogenase (GDH)-catalyzed glucose reaction. The sensor exhibits a linear glucose response range from 0.01 to 38.6 mmol/L, with a limit of detection (LOD) as low as 7.0 µmol/L and a sensitivity of 38 µA·L/(mmol·cm). The accuracy of the sensor was further validated in spiked recovery tests of human venous blood samples. The glucose recovery rate was between 99.5% and 107%, with a relative standard deviation (RSD) of less than 3.2%. These results demonstrate that our sensor has high potential for commercialization and practical application in glucose monitoring.
电化学葡萄糖传感器在监测糖尿病患者血糖水平方面具有巨大潜力。在本研究中，我们提出了一种基于1,10 - 菲咯啉 - 5,6 - 二酮（PD）/ Ru(III)作为双氧化还原介质的新型电化学葡萄糖传感器。利用PD和Ru(III)的协同效应有效地促进了酶活性中心与电极之间的电子转移。然后，基于丝网印刷电极构建了一种商用一次性电化学葡萄糖传感器。实验结果表明，PD和Ru(III)之间的协同作用为葡萄糖脱氢酶（GDH）催化的葡萄糖反应提供了良好的电子转移环境。该传感器的线性葡萄糖响应范围为0.01至38.6 mmol/L，检测限（LOD）低至7.0 µmol/L，灵敏度为38 µA·L/(mmol·cm)。在人静脉血样本的加标回收率测试中进一步验证了该传感器的准确性。葡萄糖回收率在99.5%至107%之间，相对标准偏差（RSD）小于3.2%。这些结果表明，我们的传感器在葡萄糖监测方面具有很高的商业化和实际应用潜力。
