Du Xiaoran, Zeng Yan, Li Yiying, Peng Qin, Miao Jianqiang, Liu Xili
State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, China.
Department of Plant Pathology, College of Plant Protection, China Agricultural University, 2 Yuanmingyuanxi Road, Beijing 100193, China.
J Fungi (Basel). 2024 Dec 27;11(1):12. doi: 10.3390/jof11010012.
While the rapid rise in bioinformatics has facilitated the identification of the domains and functions of many proteins, some still have no domain annotation or largely uncharacterized functions. However, the biological roles of unknown proteins were not clear in oomycetes. An analysis of the genome database identified the protein Ps495620, which has no domain annotations and functional predictions in . This study used a CRISPR/Cas9-mediated gene replacement system to knock out to elucidate its function. The -knockout mutants exhibited significantly increased oospore production and decreased sporangium formation compared to the wild-type strain P6497. Transcriptomics showed that it is a key regulator of nitrogen, pyruvate, ascorbate, and adorate metabolism in . Our findings indicate that Ps495620 is critical in regulating sporangium formation and oospore production in .
虽然生物信息学的迅速发展促进了许多蛋白质的结构域和功能的鉴定，但仍有一些蛋白质没有结构域注释或功能大多未被表征。然而，未知蛋白质在卵菌中的生物学作用尚不清楚。对基因组数据库的分析鉴定出了蛋白质Ps495620，其在……中没有结构域注释和功能预测。本研究使用CRISPR/Cas9介导的基因替换系统敲除……以阐明其功能。与野生型菌株P6497相比，……敲除突变体的卵孢子产量显著增加，孢子囊形成减少。转录组学表明，它是……中氮、丙酮酸、抗坏血酸和阿多酸代谢的关键调节因子。我们的研究结果表明，Ps495620在调节……的孢子囊形成和卵孢子产生中起关键作用。
