Xue Zhaolin, Wang Weizhen, Shen Jinghuan, Zhang Jinhui, Zhang Xitao, Liu Xili
Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, China.
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, China.
Front Microbiol. 2021 Oct 7;12:673784. doi: 10.3389/fmicb.2021.673784. eCollection 2021.
Patched (Ptc) and Patched-related (Ptr) proteins containing sterol-sensing domains (SSD) and Patched domains are highly conserved in eukaryotes for lipid transport and metabolism. Four proteins containing predicted SSD and Patched domains were simultaneously found by searching the genome database, and one of them was identified as a Patched-like (PTL) protein. Here, we investigated the biological function of . The expression level of was higher during mycelial and sporulation stages, compared to zoospore (ZO), cyst, and germinated-cyst stages, without significant change during infection. However, deletion of using CRISPR/Cas9 had no significant effect on the growth, development, or virulence of . Further investigations showed that is not essential for to cope with external stresses such as temperature, pH, oxidative and osmotic pressure. In addition, this gene did not appear to play an essential role in 's response to exogenous sterols. The transcript levels of the other three proteins containing predicted SSD and Patched domains were also not significantly upregulated in deletion transformants. Our studies demonstrated that PsPTL is not an essential protein for under the tested conditions, and more in-depth research is required for revealing the potential functions of under special conditions or in other signaling pathways.
含有固醇感应结构域（SSD）和patched结构域的patched（Ptc）蛋白及patched相关（Ptr）蛋白在真核生物中对于脂质运输和代谢高度保守。通过搜索基因组数据库同时发现了四种含有预测的SSD和patched结构域的蛋白，其中一种被鉴定为patched样（PTL）蛋白。在此，我们研究了……的生物学功能。与游动孢子（ZO）、包囊和萌发包囊阶段相比，……在菌丝体和孢子形成阶段的表达水平更高，在感染过程中无显著变化。然而，使用CRISPR/Cas9敲除……对……的生长、发育或毒力没有显著影响。进一步研究表明，……对于……应对诸如温度、pH、氧化和渗透压等外部压力并非必不可少。此外，该基因在……对外源固醇的反应中似乎也不发挥重要作用。在……敲除转化体中，其他三种含有预测的SSD和patched结构域的蛋白的转录水平也没有显著上调。我们的研究表明，在测试条件下，PsPTL对……不是必需蛋白，需要更深入的研究来揭示……在特殊条件下或其他信号通路中的潜在功能。
