Siqueira Adriele Celine, Bernardi Gisele Aparecida, Arend Lavinia Nery Villa Stangler, Cordeiro Gabrielle Tomé, Rosolen Daiane, Berti Fernanda Costa Brandão, Ferreira Amanda Maria Martins, Vasconcelos Thaís Muniz, Neves Bianca Cruz, Rodrigues Luiza Souza, Dalla-Costa Libera Maria
Instituto de Pesquisa Pelé Pequeno Príncipe (IPPPP), Curitiba 80250-060, Brazil.
Faculdades Pequeno Príncipe (FPP), Curitiba 80230-020, Brazil.
J Fungi (Basel). 2025 Jan 1;11(1):24. doi: 10.3390/jof11010024.
We investigated the molecular mechanisms underlying azole resistance in seven isolates that caused candidemia and candiduria in Paraná, Brazil (2016-2022). Biofilm production, antifungal susceptibility testing, multilocus sequence typing, amplification and sequencing of , and quantification of , , and expression levels were performed. Notably, five isolates (71.4%) were from urine samples and two (28.6%) were from blood samples. All strains were biofilm producers, with levels ranging from moderate to strong. The minimum inhibitory concentration (MIC) values ranged from 8->64 mg/L for fluconazole and 0.25-1 mg/L for voriconazole. All isolates had mutations in ; Y132F and Y257N were predominant (71.4%), followed by Y132F and S154F (14.3%) and Y257H (14.3%). No differences in expression were found between the susceptible and resistant groups, but and were more highly expressed in the susceptible isolates. All the isolates contained previously unassigned diploid sequence types. The emergence of azole resistance has been previously described in Brazil; however, the presence of resistant isolates in urine highlights the need for surveillance resistant strains in both urinary and invasive contexts. In our study mutations in were the main resistance mechanism identified in .
我们调查了巴西巴拉那州（2016 - 2022年）7株引起念珠菌血症和念珠菌尿的分离株中唑类耐药的分子机制。进行了生物膜产生、抗真菌药敏试验、多位点序列分型、[相关基因]的扩增和测序，以及[相关基因]、[相关基因]和[相关基因]表达水平的定量分析。值得注意的是，5株分离株（71.4%）来自尿液样本，2株（28.6%）来自血液样本。所有菌株均产生生物膜，水平从中度到重度不等。氟康唑的最低抑菌浓度（MIC）值范围为8 -> 64 mg/L，伏立康唑为0.25 - 1 mg/L。所有分离株在[相关基因]中均有突变；Y132F和Y257N占主导（71.4%），其次是Y132F和S154F（14.3%）以及Y257H（14.3%）。在敏感组和耐药组之间未发现[相关基因]表达的差异，但[相关基因]和[相关基因]在敏感分离株中表达更高。所有分离株均包含先前未分类的二倍体序列类型。巴西此前已有唑类耐药出现的报道；然而，尿液中耐药分离株的存在凸显了在泌尿系统和侵袭性感染环境中监测耐药菌株的必要性。在我们的研究中，[相关基因]中的突变是在[相关菌株]中鉴定出的主要耐药机制。
