Universidad San Francisco de Quito (USFQ), Colegio de Ciencias Biológicas y Ambientales COCIBA, Instituto de Microbiología, Laboratorio de Bacteriología, Quito, Ecuador.
Center of Nanoscience and Nanotechnology, Universidad de las Fuerzas Armadas (ESPE), Sangolquí, Ecuador.
Front Cell Infect Microbiol. 2022 Aug 18;12:953168. doi: 10.3389/fcimb.2022.953168. eCollection 2022.
is an emergent pathogen with a high rate of mortality associated with its biofilm formation. Biofilm formation has important repercussions on the public health system. However, little is still known about its biofilm life cycle. The present study analyzed the biofilm life cycle of and during various timepoints (24, 48, 72, and 96 h) through biomass assays, colony-forming unit (CFU) counting, and epifluorescence and scanning electron microscopies. Our results showed a significant difference between and biofilms in each biomass and viability assay. All-time samples in the biomass and viability assays confirmed statistical differences between the species through pairwise Wilcoxon tests ( < 0.05). demonstrated a lower biomass growth but reached nearly the same level of biomass at 96 h, while the CFU counting assays exhibited a superior number of viable cells within the biofilm. Statistical differences were also found between and biofilms from 48- and 72-h microscopies, demonstrating with a higher number of total cells within biofilms and cells with a superior cell area and higher matrix production. Therefore, the present study proved the higher biofilm production of .
是一种具有高死亡率的新兴病原体，其生物膜形成与其密切相关。生物膜形成对公共卫生系统有重要影响。然而，人们对其生物膜生命周期的了解仍然很少。本研究通过生物量测定、集落形成单位（CFU）计数以及荧光显微镜和扫描电子显微镜分析了 和 在不同时间点（24、48、72 和 96 小时）的生物膜生命周期。我们的结果表明，在每个生物量和存活测定中， 和 生物膜之间存在显著差异。通过两两 Wilcoxon 检验（ < 0.05），在生物量和存活测定的所有时间样本中均证实了 种间的统计学差异。 表现出较低的生物量生长，但在 96 小时时达到了几乎与 生物量相同的水平，而 CFU 计数测定显示 生物膜内具有更多的存活细胞。在 48 小时和 72 小时的显微镜观察中也发现了 和 生物膜之间的统计学差异，表明 生物膜内具有更多的总细胞，而 细胞具有更大的细胞面积和更高的基质生成。因此，本研究证明了 具有更高的生物膜生成能力。
