Kanalbek Gulzat, Zhanuzak Akniyet, Faleev Dmitry, Nusupov Aidos, Mukhatayeva Karlygash, Boguspaev Kenzhe-Karim
Faculty of Biology and Biotechnology, Al-Fabari Kazakh National University, Almaty 050040, Kazakhstan.
Faculty of Technology, Almaty Technological University, Almaty 050012, Kazakhstan.
J Fungi (Basel). 2025 Jan 7;11(1):42. doi: 10.3390/jof11010042.
The aim of the present research is the isolation and morphological and molecular-phenological identification of nematophagous fungi of Southern Kazakhstan for the production of effective bionematicides on their basis. Nematophagous fungi, which include nematode-trapping, ovicidal, endoparasitic, toxin-producing, and special substance-producing fungi, are among the most effective biological agents in controlling phytoparasitic nematodes. To isolate and characterize nematophagous fungi, soil samples were collected at 12 sites in three regions of Southern Kazakhstan. The samples were collected using the envelope method. The content of nematophagous fungi in the samples was determined using the standard surface sowing technique. The obtained strains of nematophagous fungi were identified. The attractive and nematophagous activity of the obtained fungal strains was determined by using standard methods. In experiments on the isolation and morphological identification of nematophagous fungi, the nematode species was used. Identification of the strains was carried out by the method of determining the direct nucleotide sequence of the region of the nuclear ribosomal internal transcribed spacer, followed by determination of nucleotide identity with sequences deposited in the international GeneBank database. As a result, the following species of nematophagous fungi living in the soils of agricultural lands in Southern Kazakhstan were identified: , , , and .
本研究的目的是分离哈萨克斯坦南部的捕食线虫真菌，并对其进行形态学和分子物候学鉴定，以便在此基础上生产有效的生物杀线虫剂。捕食线虫真菌包括捕食线虫、杀卵、内寄生、产毒素和产生特殊物质的真菌，是控制植物寄生线虫最有效的生物制剂之一。为了分离和鉴定捕食线虫真菌，在哈萨克斯坦南部三个地区的12个地点采集了土壤样本。样本采用信封法采集。样本中捕食线虫真菌的含量采用标准表面播种技术测定。对获得的捕食线虫真菌菌株进行了鉴定。通过标准方法测定了所获得真菌菌株的引诱和捕食线虫活性。在捕食线虫真菌的分离和形态鉴定实验中，使用了线虫物种。通过测定核糖体核糖体内转录间隔区的直接核苷酸序列，然后与国际基因库数据库中保存的序列进行核苷酸同一性测定的方法对菌株进行鉴定。结果，鉴定出了生活在哈萨克斯坦南部农田土壤中的以下捕食线虫真菌物种： 、 、 和 。
