Huang Jingshan, Wu Hongxia, Gao Tianqi, Zhai Huanjie, Moon Assad, Song Xin, Li Shuwen, Lu Zhanhao, Lan Jing, Zhong Dailang, Zhang Xinyu, Qiu Hua-Ji, Li Yongfeng, Sun Yuan
State Key Laboratory for Animal Disease Control and Prevention, National African Swine Fever Para-Reference Laboratory, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China.
College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
Vaccines (Basel). 2024 Dec 24;13(1):5. doi: 10.3390/vaccines13010005.
BACKGROUND/OBJECTIVES: African swine fever (ASF), caused by African swine fever virus (ASFV), poses a significant threat to the global swine industry. This underscores the urgent need for safe and effective ASF vaccines.
Here, we constructed five bacterium-like particles (BLPs) that each display one of the five ASFV antigens (F317L, H171R, D117L, B602L, and p54) based on the Gram-positive enhancer matrix-protein anchor (GEM-PA) system. GEM is a bacterial particle that contains only peptidoglycan, while PA is composed of three lysin motifs (Lysm) derived from the C-terminus of the AcmA protein, capable of non-covalently binding to GEM. By fusing the ASFV antigens with PA, the ASFV antigens can be firmly attached to the surface of GEM. Subsequently, the piglets were immunized via intramuscular injection with a mixture of BLPs-F317L, BLPs-H171R, BLPs-D117L, BLPs-B602L, and BLPs-p54.
The results showed that the piglets developed detectable serum IgG antibodies 2 weeks after the first immunization, and these high antibody levels were maintained 4 weeks after the booster immunization. Moreover, these piglets produced more IFN--producing lymphocytes than the control piglets.
The data indicate that the generated BLPs mixture can stimulate both humoral and cellular immune responses in piglets, these five ASFV proteins are promising antigens, and the BLPs generated represent candidate ASF vaccines.
背景/目的：由非洲猪瘟病毒（ASFV）引起的非洲猪瘟（ASF）对全球养猪业构成重大威胁。这凸显了对安全有效的ASF疫苗的迫切需求。
在此，我们基于革兰氏阳性增强子基质蛋白锚定（GEM-PA）系统构建了五种类细菌颗粒（BLP），每种颗粒展示五种ASFV抗原（F317L、H171R、D117L、B602L和p54）之一。GEM是一种仅含肽聚糖的细菌颗粒，而PA由源自AcmA蛋白C末端的三个溶素基序（Lysm）组成，能够与GEM非共价结合。通过将ASFV抗原与PA融合，ASFV抗原可牢固地附着在GEM表面。随后，通过肌肉注射用BLP-F317L、BLP-H171R、BLP-D117L、BLP-B602L和BLP-p54的混合物对仔猪进行免疫。
结果表明，仔猪在首次免疫后2周产生了可检测到的血清IgG抗体，并且在加强免疫后4周这些高抗体水平得以维持。此外，这些仔猪产生的产生干扰素的淋巴细胞比对照仔猪更多。
数据表明，所产生的BLP混合物可刺激仔猪的体液免疫和细胞免疫反应，这五种ASFV蛋白是有前景的抗原，并且所产生的BLP代表候选ASF疫苗。
