Gershoni J M, Davis F E, Palade G E
Anal Biochem. 1985 Jan;144(1):32-40. doi: 10.1016/0003-2697(85)90080-6.
Experimental data and computer mapping were used to analyze electric fields generated by a variety of electrode arrays in protein blotting apparatus. Asymmetric electrode arrays were found to generate nonuniform fields that effected uneven transfers of 125I-labeled albumin from gels to nitrocellulose membranes. Symmetric arrays with multiple (four), independent wire electrodes, supplied individually with electric current, generated the most uniform fields and effected the most even transfers of the test protein. With multiple independent electrodes, gradient electric fields can be generated in which differences in electrophoretic elution between large and small proteins can be eliminated. Transfer apparatus with either uniform or gradient electric fields are expected to improve qualitative results and make possible quantitation of protein blotting.
实验数据和计算机绘图被用于分析蛋白质印迹装置中各种电极阵列所产生的电场。发现不对称电极阵列会产生不均匀的电场，从而影响125I标记的白蛋白从凝胶到硝酸纤维素膜的不均匀转移。具有多个（四个）独立线电极且分别供电的对称阵列产生的电场最均匀，并且对测试蛋白质的转移也最均匀。使用多个独立电极，可以产生梯度电场，从而消除大蛋白质和小蛋白质之间电泳洗脱的差异。具有均匀或梯度电场的转移装置有望改善定性结果，并使蛋白质印迹的定量分析成为可能。
