Enerbäck L, Kolset S O, Kusche M, Hjerpe A, Lindahl U
Biochem J. 1985 Apr 15;227(2):661-8. doi: 10.1042/bj2270661.
Rats were infected with the nematode Nippostrongylus brasiliensis, resulting in an approx. 5-fold increase in the number of mucosal mast cells and the histamine content of the intestinal (jejunum) wall. After injection of the infected animals with inorganic [35S]sulphate, a similar increase in the yield of labelled intestinal glycosaminoglycans was observed, compared with uninfected control rats. Autoradiography showed a highly selective labelling of the numerous mucosal mast cells and of the few connective-tissue mast cells in the subserosal region of the bowel. Analysis of the labelled polysaccharide from the infected animals showed that almost 60% of this material consisted of oversulphated galactosaminoglycan, whereas heparin-related polysaccharides accounted for only 13%. The galactosaminoglycan contained 4-monosulphated and 4,6-disulphated N-acetylgalactosamine residues in approx. 5:1 molar ratio, both being linked to D-glucuronic acid residues; the occurrence of L-iduronic acid units could not be excluded. No significant difference in structure was found between this polysaccharide and the corresponding component isolated from uninfected rats. It is concluded that the major polysaccharide produced by rat mucosal mast cells in vivo is an oversulphated galactosaminoglycan rather than heparin.
将巴西日圆线虫感染大鼠，导致粘膜肥大细胞数量及肠（空肠）壁组胺含量增加约5倍。给感染动物注射无机[35S]硫酸盐后，与未感染的对照大鼠相比，观察到标记的肠糖胺聚糖产量有类似增加。放射自显影显示，肠道浆膜下区域大量的粘膜肥大细胞和少量的结缔组织肥大细胞有高度选择性标记。对感染动物的标记多糖分析表明，这种物质近60%由过度硫酸化的半乳糖胺聚糖组成，而肝素相关多糖仅占13%。半乳糖胺聚糖含有4-单硫酸化和4,6-二硫酸化的N-乙酰半乳糖胺残基，摩尔比约为5:1，两者均与D-葡萄糖醛酸残基相连；不能排除L-艾杜糖醛酸单元的存在。该多糖与从未感染大鼠分离的相应成分在结构上未发现显著差异。得出的结论是，大鼠粘膜肥大细胞在体内产生的主要多糖是过度硫酸化的半乳糖胺聚糖而非肝素。
