Eker Samet, Yaman Mustafa, Ertürk Ömer, Güvendik Tuğba Sağlam, Algı Gönül
Department of Molecular Biology and Genetics, Faculty of Arts and Science, Ordu University, Ordu, Türkiye.
Department of Biology, Faculty of Arts and Science, Bolu Abant İzzet Baysal University, 14030, Bolu, Türkiye.
Acta Parasitol. 2025 Jan 24;70(1):21. doi: 10.1007/s11686-024-00963-3.
The brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae), is an invasive and a highly polyphagous species with a strong dispersal capacity. Unfortunately, there is currently no effective control method that can prevent or reduce the economic loss caused by this pest. Among natural enemies, microsporidia cause infections in insects so that they can generally shorten life span, reduce fertility and inhibit growth.
Current study involved field study, light and electron microscopy and molecular phylogenic analyses of a microsporidian pathogen in the populations of H. halys in Türkiye.
The microsporidian infection were mostly observed in malpighian tubules and fatty tissue filled with high spore density. Fresh uninucleate spores are oval, measued as 3.73 × 2.01 µm in dimensions. The mature spore wall is relatively thick and measures 75-85 nm and consists of a clear endospore (40-50 nm) and an electron-dense, uniform, thin exospore (25-30 nm). The polar filament is isofilar, 85-110 mm in diameter and has 7-8 coils. It is found to be most closely related to Nosema maddoxi isolate Mn.6 isolated from H. halys in Georgia in phylogenetic tree.
The spore morphology and structural features of the isolate from H. halys identify it as Nosema maddoxi. The phylogenetic analyses confirm both light and electron microscopic observations. This is the first microsporidian record from H. halys and also from the order Hemiptera in Türkiye. It is also confirmed that the invasive pest, H. halys carries its natural pathogen, N. maddoxi to new geographical locations during its distribution.
褐纹蝽，Halyomorpha halys（半翅目：蝽科），是一种具有很强扩散能力的入侵性多食性物种。不幸的是，目前尚无有效的防治方法能够预防或减少这种害虫造成的经济损失。在天敌中，微孢子虫可感染昆虫，通常会缩短其寿命、降低繁殖力并抑制生长。
本研究涉及对土耳其褐纹蝽种群中一种微孢子虫病原体进行田间研究、光学和电子显微镜观察以及分子系统发育分析。
微孢子虫感染主要在马氏管和充满高孢子密度的脂肪组织中观察到。新鲜的单核孢子呈椭圆形，尺寸为3.73×2.01微米。成熟的孢子壁相对较厚，为75 - 85纳米，由一层清晰的内膜（40 - 50纳米）和一层电子致密、均匀、较薄的外膜（25 - 30纳米）组成。极丝为等丝，直径85 - 110毫米，有7 - 8个螺旋。在系统发育树中发现它与从格鲁吉亚褐纹蝽分离出的Nosema maddoxi分离株Mn.6关系最为密切。
从褐纹蝽分离出的菌株的孢子形态和结构特征将其鉴定为Nosema maddoxi。系统发育分析证实了光学和电子显微镜观察结果。这是土耳其褐纹蝽以及半翅目昆虫中首次记录到的微孢子虫。还证实了这种入侵害虫褐纹蝽在其扩散过程中将其天然病原体Nosema maddoxi携带到新的地理位置。
