在生长育肥猪舍的空气排放物中检测猪繁殖与呼吸综合征病毒2型
Detection of porcine reproductive and respiratory syndrome virus type 2 in air emissions from grow-finish barns.
作者信息
Schwartz Mark, Snow Casondra, Torremorell Montserrat, Rendahl Aaron, Pieters Maria
机构信息
Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, USA.
Schwartz Farms, Sleepy Eye, Minnesota, USA.
出版信息
Vet Rec. 2025;196(7):e5127. doi: 10.1002/vetr.5127. Epub 2025 Jan 23.
BACKGROUND
Diagnosis of porcine reproductive and respiratory syndrome virus (PRRSV) infections can be accomplished using various sample types and testing methods. The objective of this study was to evaluate the feasibility of using air emission samples to detect the onset of PRRSV type 2 infections in growing pigs.
METHODS
Air emissions and oral fluid samples were collected from three grow-finish barns, stocked with PRRSV-negative pigs every 2 weeks for 14-20 weeks. All samples were assayed for PRRSV using RT-PCR.
RESULTS
In all barns, PRRSV was initially detected in both air emissions and oral fluid samples at the same sampling event. Compared with oral fluids, air emissions collected subsequently yielded higher cycle threshold values and showed a lower likelihood of being RT-PCR positive.
LIMITATION
Sampling was limited to three grow-finish barns.
CONCLUSION
Air emission samples from pig barns are a biosecure and non-invasive sample type that is comparable to oral fluids for detection of the onset of PRRSV infection.
背景
猪繁殖与呼吸综合征病毒（PRRSV）感染的诊断可通过多种样本类型和检测方法来完成。本研究的目的是评估利用空气排放样本检测生长猪群中2型PRRSV感染起始情况的可行性。
方法
从三个育成猪舍采集空气排放样本和口腔液体样本，这些猪舍饲养的猪PRRSV检测呈阴性，每2周采集一次样本，共采集14 - 20周。所有样本均采用逆转录聚合酶链反应（RT-PCR）检测PRRSV。
结果
在所有猪舍中，最初在同一采样时间点的空气排放样本和口腔液体样本中均检测到PRRSV。与口腔液体相比，随后采集的空气排放样本产生的循环阈值更高，且RT-PCR检测呈阳性的可能性更低。
局限性
采样仅限于三个育成猪舍。
结论
猪舍的空气排放样本是一种生物安全且非侵入性的样本类型，在检测PRRSV感染起始情况方面与口腔液体相当。
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