Wang Jing, Huang Zenan, Han Zhenzhong, Luan Jing, Li Zihan, Guo Xutong, Yang Dongxu, Cui Yazhou, Han Jinxiang, Xu Duo
Department of Orthopedic Surgery, The First Affiliated Hospital of Shandong First Medical University, Ji'nan, Shandong, 250014, China.
Biomedical Sciences College & Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji'nan, Shandong, 250117, China.
Adv Healthc Mater. 2025 Jan 24:e2404768. doi: 10.1002/adhm.202404768.
The porous structure is crucial in bone tissue engineering for promoting osseointegration. Among various structures, triply periodic minimal surfaces (TPMS) -Gyroid has been extensively studied due to its superior mechanical and biological properties. However, previous studies have given limited attention to the impact of unit cell size on the biological performance of scaffolds. In this research, four TPMS-Gyroid titanium scaffolds with different unit cell sizes (TG15, TG20, TG25, and TG30) are fabricated using Selective Laser Melting (SLM) to explore their effects on osseointegration. Mechanical tests revealed that TG15 and TG20 exhibited superior compressive strength. In vitro experiments demonstrated that TG20 facilitated better cell adhesion through robust integrin protein expression initially, which subsequently enhanced cell proliferation and osteogenic differentiation. Furthermore, macrophages on TG20 showed higher Integrin β1 (ITGB1) expression, promoting their polarization to the M2 phenotype, which suppressed inflammation, fostered bone integration, and angiogenesis. In vivo studies confirmed TG20's effectiveness in promoting bone ingrowth by reducing inflammation. This study highlights TG20's structural advantages, making it a promising bone scaffold with exceptional osteogenic and angiogenic properties through osteoimmune microenvironment modulation. Therefore, TG20 holds significant potential for applications in bone tissue engineering.
多孔结构在促进骨整合的骨组织工程中至关重要。在各种结构中，三重周期极小曲面（TPMS）-类螺旋面因其优异的力学和生物学性能而受到广泛研究。然而，以往的研究对晶胞尺寸对支架生物学性能的影响关注有限。在本研究中，使用选择性激光熔化（SLM）制造了四种具有不同晶胞尺寸的TPMS-类螺旋面钛支架（TG15、TG20、TG25和TG30），以探讨它们对骨整合的影响。力学测试表明，TG15和TG20表现出优异的抗压强度。体外实验表明，TG20最初通过强大的整合素蛋白表达促进了更好的细胞黏附，随后增强了细胞增殖和成骨分化。此外，TG20上的巨噬细胞显示出更高的整合素β1（ITGB1）表达，促进它们向M2表型极化，从而抑制炎症、促进骨整合和血管生成。体内研究证实了TG20通过减轻炎症促进骨向内生长的有效性。本研究突出了TG20的结构优势，使其成为一种有前途的骨支架，通过调节骨免疫微环境具有卓越的成骨和血管生成特性。因此，TG20在骨组织工程应用中具有巨大潜力。
