Bassan Tiziana, Pastor Josep, Martinez-Caro Javier, Agulla Beatriz, Martorell Jaume
Fundació Hospital Clínic Veterinari, Facultat de Veterinària, Universitat Autònoma de Barcelona, Cerdanyola del Vallès 08193, Spain; Departament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, Cerdanyola del Vallès 08193, Spain.
Departament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, Cerdanyola del Vallès 08193, Spain.
Vet J. 2025 Apr;310:106298. doi: 10.1016/j.tvjl.2025.106298. Epub 2025 Jan 22.
Blood sampling and analysis are essential procedures for assessing the health status of exotic pets. While careful manual restraint is generally recommended, sedation may be necessary in specific cases. However, the use of chemical restraint may introduce analytical variations. The aim of this study is to determine the effects of sedation with butorphanol, midazolam, ketamine and dexmedetomidine on haematological, biochemical and coagulation parameters (prothrombin time (PT), activated partial thromboplastin time (aPTT), and fibrinogen), as well as thromboelastography in adult healthy New Zealand rabbits (Oryctolagus cuniculus). Two groups of adult New Zealand rabbits, housed under identical conditions and considered healthy based on normal physical examination and unremarkable clinical histories, were included in the study. The non-sedated group comprised ten rabbits, while the sedated group consisted of fifteen. Blood samples were collected via jugular venipuncture, and haematological, biochemical and coagulation profiles were performed. In conclusion, some variation in haematological and biochemical values were observed depending on sex and/or sedation protocols. Sex can influence haematology in terms of haemoglobin concentration, lymphocyte and platelet count; biochemistry in alanine aminotransferase, chloride, gamma-glutamyl transferase, potassium, sodium, calcium, total cholesterol, urea nitrogen, but not in coagulation parameters. Meanwhile, sedation can affect haematology in terms of leucocyte and lymphocyte count and biochemistry in total bilirubin, calcium, total protein, sodium, albumin, glucose, creatinine, phosphorous. Plasma-based coagulation assays showed increased prolongation of aPTT and PT, although these changes do not seem to be clinically relevant. There were no changes observed in thromboelastographic parameters.
血液采样和分析是评估外来宠物健康状况的重要程序。虽然通常建议进行仔细的手动约束，但在特定情况下可能需要镇静。然而，使用化学约束可能会引入分析差异。本研究的目的是确定布托啡诺、咪达唑仑、氯胺酮和右美托咪定镇静对成年健康新西兰兔（穴兔）血液学、生化和凝血参数（凝血酶原时间（PT）、活化部分凝血活酶时间（aPTT）和纤维蛋白原）以及血栓弹力图的影响。两组成年新西兰兔被纳入研究，它们在相同条件下饲养，根据正常体格检查和无异常临床病史被认为是健康的。未镇静组包括10只兔子，而镇静组由15只组成。通过颈静脉穿刺采集血样，并进行血液学、生化和凝血分析。总之，根据性别和/或镇静方案观察到血液学和生化值存在一些差异。性别可在血红蛋白浓度、淋巴细胞和血小板计数方面影响血液学；在丙氨酸氨基转移酶、氯、γ-谷氨酰转移酶、钾、钠、钙、总胆固醇、尿素氮方面影响生化，但对凝血参数无影响。同时，镇静可在白细胞和淋巴细胞计数方面影响血液学，在总胆红素、钙、总蛋白、钠、白蛋白、葡萄糖、肌酐、磷方面影响生化。基于血浆的凝血试验显示aPTT和PT延长增加，尽管这些变化似乎与临床无关。血栓弹力图参数未观察到变化。
