Volpato Julieta, Mattoso Cláudio Roberto Scabelo, Beier Suzane Lilian, Coelho Mirelly Medeiros, Tocheto Ronise, Kirsten Cristine Elizabeth, Yonezawa Letícia Andreza, Saito Mere Erika
Department of Veterinary Medicine, Agroveterinary Sciences Center, Santa Catarina State University (UDESC), Av. Luiz de Camoes, 2090, Conta Dinheiro, 88520-000, Lages, SC, Brazil
Department of Veterinary Medicine, Agroveterinary Sciences Center, Santa Catarina State University (UDESC), Av. Luiz de Camoes, 2090, Conta Dinheiro, 88520-000, Lages, SC, Brazil.
J Feline Med Surg. 2015 Jun;17(6):500-6. doi: 10.1177/1098612X14549214. Epub 2014 Sep 12.
Acute stress induced by physical restraint can interfere with the validity of laboratory findings. Sedation could minimize such stress. However, it is not known whether sedation can affect hematologic and hemostatic parameters in cats. The purpose of this study was to evaluate hematologic and hemostatic parameters in domestic cats subjected to physical restraint in addition to one of two sedation protocols. In total, 50 cats were subjected to physical restraint and were then randomly divided into two groups of 25 animals, receiving dexmedetomidine (5 µg/kg) and butorphanol (0.3 mg/kg; DB group) or dexmedetomidine (5 µg/kg), butorphanol (0.3 mg/kg) and ketamine (3 mg/kg; DBK group). The cats were assessed for acute stress, sedation level, onset of sedation and duration of sedation. Blood samples were collected after handling and after sedation. The complete blood count (CBC), platelet count, buccal mucosal bleeding time (BMBT), whole-blood clotting time, prothrombin time (PT), activated partial thromboplastin time (aPTT) and thrombin time (TT) were determined for each sample, before and after chemical restraint. No statistically significant differences were found in the hematologic parameters. Certain hemostatic parameters (PT, aPTT and TT) were higher in the DB group (P <0.05). The onset of sedation was similar in the two groups, and the duration of sedation was longer in the DBK group. Both sedation protocols were effective for short-duration chemical restraint for blood collection from the studied cats, and no clinically relevant effects on hematologic or hemostatic parameters were detected.
身体束缚诱导的急性应激会干扰实验室检查结果的有效性。镇静可将此类应激降至最低。然而，尚不清楚镇静是否会影响猫的血液学和止血参数。本研究的目的是评估在两种镇静方案之一的基础上接受身体束缚的家猫的血液学和止血参数。总共50只猫接受了身体束缚，然后随机分为两组，每组25只动物，分别接受右美托咪定（5μg/kg）和布托啡诺（0.3mg/kg；DB组）或右美托咪定（5μg/kg）、布托啡诺（0.3mg/kg）和氯胺酮（3mg/kg；DBK组）。对猫的急性应激、镇静水平、镇静起效时间和镇静持续时间进行评估。在处理后和镇静后采集血样。在化学束缚前后，对每个样本测定全血细胞计数（CBC）、血小板计数、口腔黏膜出血时间（BMBT）、全血凝固时间、凝血酶原时间（PT）、活化部分凝血活酶时间（aPTT）和凝血酶时间（TT）。血液学参数未发现统计学上的显著差异。DB组某些止血参数（PT、aPTT和TT）较高（P<0.05）。两组的镇静起效时间相似，DBK组的镇静持续时间更长。两种镇静方案对从受试猫采集血液的短期化学束缚均有效，且未检测到对血液学或止血参数有临床相关影响。
