Rege V
Ann Clin Biochem. 1985 Jan;22 ( Pt 1):98-103. doi: 10.1177/000456328502200112.
Commercial RIA kits for the assay of serum total oestriol using 125I label are used widely in monitoring fetal well-being in pregnancy; they are, however, very expensive for large-scale routine use. An 'in-house' assay using commercially available [125I]oestriol label and antiserum produced by the S.E. and S.W. Thames Regional Antibody Production Unit has been developed. Technical aspects of the method are described. The assay is compared with two commercial kits and correlates very well with one of them. The assay gives good precision and can be used with substantial savings in cost.
使用125I标记的用于测定血清总雌三醇的商业放射免疫分析试剂盒在监测孕期胎儿健康方面被广泛应用；然而，对于大规模常规使用来说，它们非常昂贵。一种使用市售[125I]雌三醇标记和由东南泰晤士河及西南泰晤士河地区抗体生产单位生产的抗血清的“内部”分析方法已经开发出来。描述了该方法的技术方面。将该分析方法与两种商业试剂盒进行了比较，并且与其中一种试剂盒的相关性非常好。该分析方法具有良好的精密度，并且使用时能大幅节省成本。
