Kerr E J, Park B K, Dean P D
Clin Chim Acta. 1977 May 16;77(1):77-82. doi: 10.1016/0009-8981(77)90404-1.
A specific radioimmunoassay has been developed for measuring oestriol-16alpha glucuronide in pregnancy plasma using a highly specific antiserum. The specificity of this antiserum has been assessed by the 50% displacement method and by measuring oestriol-16alpha-glucuronide concentrations in pregnancy plasma samples, both with and without chromatography. The antiserum was found to have Ka of 1.3 X 10(10) M-1. The assay had a sensitivity of 5 pg (least quantity of oestriol-16alpha-glucuronide distinguishable from the zero point, p less than 0.05), an intra-assay variation of 6.07% and an inter-assay variation of 3.36%. The assay was used to measure plasma oestriol-16alpha-glucuronide concentrations throughout eight pregnancies, five of which were normal and three had some defined abnormality.
已开发出一种特异性放射免疫分析法，使用高度特异性抗血清来测量妊娠血浆中的雌三醇 - 16α - 葡萄糖醛酸苷。该抗血清的特异性已通过50%置换法以及测量有无色谱法处理的妊娠血浆样本中雌三醇 - 16α - 葡萄糖醛酸苷浓度进行评估。发现该抗血清的亲和常数（Ka）为1.3×10¹⁰ M⁻¹。该分析方法的灵敏度为5皮克（可与零点区分开的雌三醇 - 16α - 葡萄糖醛酸苷的最小量，p < 0.05），批内变异为6.07%，批间变异为3.36%。该分析方法用于测量八次妊娠期间的血浆雌三醇 - 16α - 葡萄糖醛酸苷浓度，其中五次妊娠正常，三次有明确异常。
