Wray S K, Gilbert B E, Knight V
Antiviral Res. 1985 Feb;5(1):39-48. doi: 10.1016/0166-3542(85)90013-0.
These studies examine the effect of ribavirin triphosphate (RTP) on two replicative functions associated with influenza virus nucleocapsids, primer generation and its subsequent elongation. To study primer generation influenza virus cores were added to beta-globin mRNA in the presence of only [32P]GTP. To examine elongation, ATP and CTP were added to the reaction mixture to permit limited elongation, and products from both reactions were separated on polyacrylamide gels and quantified. Under these conditions, the 50% inhibitory concentration of RTP for primer generation was 3.0 mM, and the 50% inhibitory concentration for elongation was 0.6 mM. RNA polymerase activity associated with cores isolated from clinical strains of influenza A and B viruses reacted as did the laboratory strain of influenza virus and was equally susceptible to inhibition by RTP.
这些研究考察了三磷酸利巴韦林（RTP）对与流感病毒核衣壳相关的两种复制功能的影响，即引物生成及其后续延伸。为了研究引物生成，仅在存在[32P]GTP的情况下将流感病毒核心添加到β-珠蛋白mRNA中。为了检测延伸，将ATP和CTP添加到反应混合物中以允许有限的延伸，并且两个反应的产物在聚丙烯酰胺凝胶上分离并定量。在这些条件下，RTP对引物生成的50%抑制浓度为3.0 mM，对延伸的50%抑制浓度为0.6 mM。与从甲型和乙型流感病毒临床分离株中分离出的核心相关的RNA聚合酶活性与流感病毒实验室株的反应相同，并且同样易受RTP的抑制。
