三磷酸利巴韦林对流感病毒核糖核酸聚合酶的抑制作用。
Inhibition of influenza virus ribonucleic acid polymerase by ribavirin triphosphate.
作者信息
Eriksson B, Helgstrand E, Johansson N G, Larsson A, Misiorny A, Norén J O, Philipson L, Stenberg K, Stening G, Stridh S, Oberg B
出版信息
Antimicrob Agents Chemother. 1977 Jun;11(6):946-51. doi: 10.1128/AAC.11.6.946.
Abstract
Ribavirin 5'-triphosphate (RTP), derived from the broad-spectrum antiviral compound ribavirin (Virazole), can selectively inhibit influenza virus ribonucleic acid polymerase in a cell-free assay. Ribavirin and its 5'-monophosphate have no effect on the polymerase. The inhibition is competitive with respect to adenosine 5'-triphosphate and guanosine 5'-triphosphate. RTP also inhibits ApG- and GpC-stimulated influenza virus ribonucleic acid polymerase. Since ribavirin is phosphorylated in the cell, the inhibition of influenza multiplication in the cell may also be caused by RTP.
摘要
利巴韦林5'-三磷酸（RTP）由广谱抗病毒化合物利巴韦林（病毒唑）衍生而来，在无细胞试验中可选择性抑制流感病毒核糖核酸聚合酶。利巴韦林及其5'-单磷酸对该聚合酶无作用。这种抑制作用在三磷酸腺苷和三磷酸鸟苷方面具有竞争性。RTP还抑制ApG和GpC刺激的流感病毒核糖核酸聚合酶。由于利巴韦林在细胞内被磷酸化，细胞内流感增殖的抑制也可能由RTP引起。
相似文献
1
Inhibition of influenza virus ribonucleic acid polymerase by ribavirin triphosphate.三磷酸利巴韦林对流感病毒核糖核酸聚合酶的抑制作用。
Antimicrob Agents Chemother. 1977 Jun;11(6):946-51. doi: 10.1128/AAC.11.6.946.
2
3
[Inhibitory action of adenylyl-[3'--5']-ribavirin on the RNA polymerase activity of influenza A viruses].[腺苷酰-[3'-5']-利巴韦林对甲型流感病毒RNA聚合酶活性的抑制作用]
Dokl Akad Nauk SSSR. 1984;274(4):975-8.
4
[Inhibitory effect of 3'-azido-3'-azido-3'-deoxy- ribavirin-5'-triphosphate on RNA synthesis catalyzed by RNA polymerase from influenza A virus and by cellular DNA-dependent RNA polymerase II].[3'-叠氮-3'-叠氮-3'-脱氧-利巴韦林-5'-三磷酸对甲型流感病毒RNA聚合酶及细胞DNA依赖性RNA聚合酶II催化的RNA合成的抑制作用]
Vopr Med Khim. 1989 Jan-Feb;35(1):104-6.
5
Inhibition of influenza RNA synthesis by virazole (ribavirin).病毒唑（利巴韦林）对流感病毒RNA合成的抑制作用。
Arch Virol. 1976;50(4):349-52. doi: 10.1007/BF01317961.
6
Molecular analysis of the inhibitory effect of phosphorylated ribavirin on the vesicular stomatitis virus in vitro polymerase reaction.磷酸化利巴韦林对水疱性口炎病毒体外聚合酶反应抑制作用的分子分析
Antimicrob Agents Chemother. 1989 Oct;33(10):1668-73. doi: 10.1128/AAC.33.10.1668.
7
Effect of ribavirin triphosphate on primer generation and elongation during influenza virus transcription in vitro.三磷酸利巴韦林对流感病毒体外转录过程中引物生成和延伸的影响。
Antiviral Res. 1985 Feb;5(1):39-48. doi: 10.1016/0166-3542(85)90013-0.
8
Effects of selenazofurin and ribavirin and their 5'-triphosphates on replicative functions of influenza A and B viruses.硒唑嘌呤和利巴韦林及其5'-三磷酸酯对甲型和乙型流感病毒复制功能的影响。
Antimicrob Agents Chemother. 1986 Jan;29(1):67-72. doi: 10.1128/AAC.29.1.67.
9
The ambiguous base-pairing and high substrate efficiency of T-705 (Favipiravir) Ribofuranosyl 5'-triphosphate towards influenza A virus polymerase.T-705（法匹拉韦）核糖呋喃基 5'-三磷酸对流感病毒聚合酶的碱基配对不明确和高底物效率。
PLoS One. 2013 Jul 10;8(7):e68347. doi: 10.1371/journal.pone.0068347. Print 2013.
10
Studies on the mechanism of the antiviral activity of ribavirin against reovirus.利巴韦林抗呼肠孤病毒活性机制的研究
Virology. 1989 Jan;168(1):147-58. doi: 10.1016/0042-6822(89)90413-3.
引用本文的文献
1
Ribavirin inhibits peste des petits ruminants virus proliferation .利巴韦林抑制小反刍兽疫病毒增殖。
Vet Med (Praha). 2023 Dec 26;68(12):464-476. doi: 10.17221/56/2023-VETMED. eCollection 2023 Dec.
2
Ribavirin inhibits the replication of infectious bursal disease virus predominantly through depletion of cellular guanosine pool.利巴韦林主要通过消耗细胞鸟苷池来抑制传染性法氏囊病病毒的复制。
Front Vet Sci. 2023 Jul 31;10:1192583. doi: 10.3389/fvets.2023.1192583. eCollection 2023.
3
Screening of SARS-CoV-2 antivirals through a cell-based RNA-dependent RNA polymerase (RdRp) reporter assay.通过基于细胞的RNA依赖性RNA聚合酶（RdRp）报告基因检测筛选严重急性呼吸综合征冠状病毒2（SARS-CoV-2）抗病毒药物。
Cell Insight. 2022 Jun 29;1(4):100046. doi: 10.1016/j.cellin.2022.100046. eCollection 2022 Aug.
4
Anti-influenza Virus Activity of Methylthio-Formycin Distinct From That of T-705.甲硫基嘌呤霉素与T-705不同的抗流感病毒活性。
Front Microbiol. 2022 Feb 18;13:802671. doi: 10.3389/fmicb.2022.802671. eCollection 2022.
5
Antiviral Activity of Ribavirin against in Fish Cells.利巴韦林对鱼类细胞中[病毒名称未给出]的抗病毒活性
Pathogens. 2021 Dec 10;10(12):1616. doi: 10.3390/pathogens10121616.
6
Improving properties of the nucleobase analogs T-705/T-1105 as potential antiviral.改善核碱基类似物T-705/T-1105作为潜在抗病毒药物的性能。
Annu Rep Med Chem. 2021;57:1-47. doi: 10.1016/bs.armc.2021.08.002. Epub 2021 Oct 28.
7
SARS-CoV-2-host proteome interactions for antiviral drug discovery.SARS-CoV-2 宿主蛋白组与抗病毒药物发现的相互作用。
Mol Syst Biol. 2021 Nov;17(11):e10396. doi: 10.15252/msb.202110396.
8
SARS-CoV-2: Pathogenic Mechanisms and Host Immune Response.SARS-CoV-2：致病机制与宿主免疫应答。
Adv Exp Med Biol. 2021;1313:99-134. doi: 10.1007/978-3-030-67452-6_6.
9
Development of a method for evaluating the mRNA transcription activity of influenza virus RNA-dependent RNA polymerase through real-time reverse transcription polymerase chain reaction.建立一种通过实时逆转录聚合酶链反应评估流感病毒 RNA 依赖性 RNA 聚合酶 mRNA 转录活性的方法。
Virol J. 2021 Aug 28;18(1):177. doi: 10.1186/s12985-021-01644-7.
10
Controversies' clarification regarding ribavirin efficacy in measles and coronaviruses: Comprehensive therapeutic approach strictly tailored to COVID-19 disease stages.关于利巴韦林在麻疹和冠状病毒治疗中疗效的争议澄清：针对COVID-19疾病阶段严格定制的综合治疗方法。
World J Clin Cases. 2021 Jul 6;9(19):5135-5178. doi: 10.12998/wjcc.v9.i19.5135.
本文引用的文献
1
Polyacrylamide gel electrophoresis of influenza virus RNA.流感病毒RNA的聚丙烯酰胺凝胶电泳
Virology. 1968 Feb;34(2):385-8. doi: 10.1016/0042-6822(68)90257-2.
2
Animal DNA-dependent RNA polymerases. 1. Large-scale solubilization and separation of A and B calf-thymus RNA-polymerase activities.动物依赖DNA的RNA聚合酶。1. 小牛胸腺RNA聚合酶A和B活性的大规模溶解与分离。
Eur J Biochem. 1972 Jul 13;28(2):269-76. doi: 10.1111/j.1432-1033.1972.tb01910.x.
3
4
A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.一种大规模纯化大肠杆菌脱氧核糖核酸依赖性核糖核酸聚合酶的新方法。
J Biol Chem. 1969 Nov 25;244(22):6160-7.
5
Sea urchin nuclear DNA polymerase.海胆核DNA聚合酶。
Methods Enzymol. 1974;29:53-70. doi: 10.1016/0076-6879(74)29009-8.
6
Design, synthesis, and broad spectrum antiviral activity of 1- -D-ribofuranosyl-1,2,4-triazole-3-carboxamide and related nucleosides.1-β-D-呋喃核糖基-1,2,4-三唑-3-甲酰胺及相关核苷的设计、合成与广谱抗病毒活性
J Med Chem. 1972 Nov;15(11):1150-4. doi: 10.1021/jm00281a014.
7
Broad-spectrum antiviral activity of Virazole: 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide.病毒唑的广谱抗病毒活性：1-β-D-呋喃核糖基-1,2,4-三唑-3-甲酰胺。
Science. 1972 Aug 25;177(4050):705-6. doi: 10.1126/science.177.4050.705.
8
Genome homology of vesicular stomatitis virus and defective T particles and evidence for the sequential transcription of the virion ribonucleic acid.水泡性口炎病毒与缺陷T颗粒的基因组同源性及病毒体核糖核酸顺序转录的证据。
J Virol. 1972 Jun;9(6):946-55. doi: 10.1128/JVI.9.6.946-955.1972.
9
Transcription of the influenza ribonucleic acid genome by a virion polymerase. I. Optimal conditions for in vitro activity of the ribonucleic acid-dependent ribonucleic acid polymerase.流感病毒核糖核酸基因组由病毒粒子聚合酶进行转录。I. 核糖核酸依赖性核糖核酸聚合酶体外活性的最佳条件。
J Virol. 1971 Jul;8(1):66-73. doi: 10.1128/JVI.8.1.66-73.1971.
10
Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion.水泡性口炎病毒的核糖核酸合成，II。病毒粒子中的一种RNA聚合酶。
Proc Natl Acad Sci U S A. 1970 Jun;66(2):572-6. doi: 10.1073/pnas.66.2.572.
Zotero 插件