Effect of Indigenous Non- Yeasts on Lipid Compositions of Maraština Wine.

This study is the first to investigate the impact of indigenous non-Saccharomyces yeasts, including , /, , , , , , , , and on the lipid composition of sterile Maraština grape juice and wines using the UHPLC-MS/MS method. Yeasts were tested in monoculture and sequential fermentations alongside commercial . Indigenous non-Saccharomyces yeasts showed the potential to improve fermentation performance and enable the development of new wine styles through the biosynthesis of an unsaturated fatty acid pathway, which was identified as the most significant pathway. In monoculture fermentations, , , , , and significantly reduced lignoceric acid, potentially influencing wine aroma through the formation of esters and higher alcohols. , , , , and increased the demand for lipids, such as stearic acid, which may help preserve membrane permeability by integrating into the membrane in response to ethanol shock. The most significant impact on free fatty esters was observed in fermentations with . Furthermore, in sequential fermentations significantly reduced arachidic, stearic, and palmitic acid. reduced the content of erucic and linoleic acid.