Alizadeh Bahmani Amir Hossein, Abdel-Aziz Mahmoud I, Hashimoto Simone, Bang Corinna, Brandstetter Susanne, Corcuera-Elosegui Paula, Franke Andre, Gorenjak Mario, Harner Susanne, Kheiroddin Parastoo, López-Fernández Leyre, Neerincx Anne H, Pino-Yanes Maria, Potočnik Uroš, Sardón-Prado Olaia, Toncheva Antoaneta A, Wolff Christine, Kabesch Michael, Kraneveld Aletta D, Vijverberg Susanne J H, Maitland-van der Zee Anke H
Department of Pulmonary Medicine, Amsterdam UMC Location University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands.
Amsterdam Institute for Infection and Immunity, Inflammatory Diseases, 1105 AZ Amsterdam, The Netherlands.
Biomedicines. 2025 Jan 2;13(1):89. doi: 10.3390/biomedicines13010089.
: Metered-dose inhalers (MDIs) and dry powder inhalers (DPIs) are common inhaled corticosteroid (ICS) inhaler devices. The difference in formulation and administration technique of these devices may influence oral cavity microbiota composition. We aimed to compare the saliva microbiome in children with moderate-to-severe asthma using ICS via MDIs versus DPIs. : Saliva samples collected from 143 children (6-17 yrs) with moderate-to-severe asthma across four European countries (The Netherlands, Germany, Spain, and Slovenia) as part of the SysPharmPediA cohort were subjected to 16S rRNA sequencing. The microbiome was compared using global diversity (α and β) between two groups of participants based on inhaler devices (MDI (n = 77) and DPI (n = 65)), and differential abundance was compared using the Analysis of Compositions of Microbiomes with the Bias Correction (ANCOM-BC) method. : No significant difference was observed in α-diversity between the two groups. However, β-diversity analysis revealed significant differences between groups using both Bray-Curtis and weighted UniFrac methods (adjusted -value = 0.015 and 0.044, respectively). Significant differential abundance between groups, with higher relative abundance in the MDI group compared to the DPI group, was detected at the family level [Carnobacteriaceae (adjusted = 0.033)] and at the genus level [ (adjusted = 0.021) and (adjusted = 0.011)]. : Types of ICS devices are associated with different saliva microbiome compositions in moderate-to-severe pediatric asthma. The causal relation between inhaler types and changes in saliva microbiota composition needs to be further evaluated, as well as whether this leads to different potential adverse effects in terms of occurrence and level of severity.
定量吸入器（MDIs）和干粉吸入器（DPIs）是常见的吸入性糖皮质激素（ICS）吸入装置。这些装置在制剂和给药技术上的差异可能会影响口腔微生物群的组成。我们旨在比较中度至重度哮喘儿童使用MDIs与DPIs吸入ICS时的唾液微生物群。
作为SysPharmPediA队列研究的一部分，从四个欧洲国家（荷兰、德国、西班牙和斯洛文尼亚）的143名6至17岁中度至重度哮喘儿童中收集唾液样本，并进行16S rRNA测序。根据吸入装置（MDI组n = 77，DPI组n = 65），使用全局多样性（α和β）对两组参与者的微生物群进行比较，并使用带偏差校正的微生物群组成分析（ANCOM-BC）方法比较差异丰度。
两组之间在α多样性上未观察到显著差异。然而，β多样性分析显示，使用Bray-Curtis和加权UniFrac方法时，两组之间存在显著差异（校正P值分别为0.015和0.044）。在科水平上[肉杆菌科（校正P = 0.033）]和属水平上[某属（校正P = 0.021）和另一属（校正P = 0.011）]检测到两组之间存在显著的差异丰度，MDI组的相对丰度高于DPI组。
在中度至重度儿童哮喘中，ICS装置类型与不同的唾液微生物群组成相关。吸入器类型与唾液微生物群组成变化之间的因果关系需要进一步评估，以及这是否会导致在发生和严重程度方面不同的潜在不良反应。
