Zhao Min, Zou Yu, Chen Wanting, Wu Dongqi, Xian Chengjun, Yang Haoqing, Tan Jiacheng, Di Wenda, Wu Wende, Wang Dongying
Guangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, College of Animal Science and Technology, Guangxi University, Nanning 530005, China.
Animals (Basel). 2025 Jan 10;15(2):179. doi: 10.3390/ani15020179.
can modulate host immune mechanisms through excretory-secretory products (ESP). As one of the components of ESP, it is unknown whether Abelson tyrosine protein kinase (Abl) is involved in parasite-host immune interaction. To investigate the immunoregulatory function of Abl in , we cloned and expressed the gigantica Abl protein and assessed its effect on specific immune functions of buffalo peripheral blood mononuclear cells (PBMCs). Recombinant Abelson tyrosine protein kinase (rAbl) was expressed in . Western blot analysis was performed to assess the reactivity of anti-rAbl antibodies with rAbl, serum from -infected buffalo, and excretion and secretion products of . Immunohistochemical analysis was conducted to determine the localization of Abl in tissues from larval stages and adult worms of . Furthermore, immunofluorescence analysis was utilized to evaluate the binding ability of the rAbl protein to buffalo peripheral blood mononuclear cells (PBMCs), as well as to investigate the effects of varying concentrations of rAbl protein (5, 10, 20, 40, and 80 μg/mL) on the functional responses of PBMCs. Anti-rAbl antibodies specifically recognize rAbl, serum from buffalo infected with , and ESP. rAbl is localized in the cecum and capsule of juvenile worms, as well as in the testis and viellaria of adult worms. Additionally, rAbl enhances cell proliferation, migration, nitric oxide (NO) production, and phagocytosis, while also increasing the transcription levels of cytokines (IFN-γ, IL-12, TNF-α, IL-4, IL-10, and TGF-β). The results indicate that rAbl can influence the immune function of PBMCs. Further investigation into the immunomodulatory properties of the rAbl protein will enhance our understanding of the immune interaction mechanisms between trematodes and their hosts.
可通过排泄-分泌产物（ESP）调节宿主免疫机制。作为ESP的组成成分之一，艾布勒逊酪氨酸蛋白激酶（Abl）是否参与寄生虫与宿主的免疫相互作用尚不清楚。为研究Abl在[寄生虫名称未给出]中的免疫调节功能，我们克隆并表达了大片吸虫Abl蛋白，并评估其对水牛外周血单个核细胞（PBMCs）特异性免疫功能的影响。重组艾布勒逊酪氨酸蛋白激酶（rAbl）在[表达系统未给出]中表达。进行蛋白质免疫印迹分析以评估抗rAbl抗体与rAbl、感染[寄生虫名称未给出]的水牛血清以及[寄生虫名称未给出]的排泄和分泌产物的反应性。进行免疫组织化学分析以确定Abl在[寄生虫名称未给出]幼虫期和成虫组织中的定位。此外，利用免疫荧光分析评估rAbl蛋白与水牛外周血单个核细胞（PBMCs）的结合能力，以及研究不同浓度的rAbl蛋白（5、10、20、40和80μg/mL）对PBMCs功能反应的影响。抗rAbl抗体能特异性识别rAbl、感染[寄生虫名称未给出]的水牛血清以及ESP。rAbl定位于幼虫的盲肠和包囊，以及成虫的睾丸和卵黄腺。此外，rAbl可增强细胞增殖、迁移、一氧化氮（NO）产生和吞噬作用，同时还增加细胞因子（IFN-γ、IL-12、TNF-α、IL-4、IL-10和TGF-β）的转录水平。结果表明rAbl可影响PBMCs的免疫功能。对rAbl蛋白免疫调节特性的进一步研究将增进我们对吸虫与其宿主之间免疫相互作用机制的理解。
