Rosato Roberto, Quaranta Gianluca, Santarelli Giulia, Fancello Giovanni, Bianco Delia Mercedes, Monzo Francesca Romana, Bibbò Stefano, Cammarota Giovanni, Sanguinetti Maurizio, Masucci Luca, De Maio Flavio
Department of Basic Biotechnological Sciences, Intensivology and Perioperative Clinics, Section of Microbiology, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.
Department of Laboratory and Infectious Sciences, Fondazione Policlinico Universitario "A. Gemelli" IRCCS, 00168 Rome, Italy.
Microorganisms. 2024 Dec 25;13(1):16. doi: 10.3390/microorganisms13010016.
A diverse and well-functioning gut microbiota normally serves as a protective shield against the invasion of harmful bacteria or the proliferation of opportunistic pathogens. infection (CDI) is predominantly associated with the overuse of antibiotics, resulting in a significant alteration in the gut's microbial balance. Unfortunately, the lack of global standardization does not allow for the identification of a set of biomarkers associated with the onset and progression of this disease. In this study, we examined the composition of the gut microbiota in patients at the time of the initial detection of CDI compared to a control group of CDI-negative individuals, with a focus on identifying potential CDI biomarkers for diagnosis. While no significant differences in the alpha and beta diversity between CDI-negative and CDI-positive individuals were found, we found that certain genera (such as XIVa and XVIII) showed different abundance patterns in the two groups, indicating potential differences in gut microbiota balance. In conclusion, am enrichment in XI and a decrease in emerged in the CDI-positive patients and following antibiotic treatment, indicating that changes in the ratio may be a promising biomarker that warrants further investigation for CDI diagnosis.
多样且功能良好的肠道微生物群通常可作为抵御有害细菌入侵或机会性病原体增殖的保护屏障。艰难梭菌感染（CDI）主要与抗生素的过度使用有关，这会导致肠道微生物平衡发生显著改变。不幸的是，缺乏全球标准化使得无法确定一组与该疾病的发生和进展相关的生物标志物。在本研究中，我们检查了CDI初次检测时患者的肠道微生物群组成，并与CDI阴性个体的对照组进行比较，重点是识别用于诊断的潜在CDI生物标志物。虽然在CDI阴性和CDI阳性个体之间未发现α和β多样性的显著差异，但我们发现某些菌属（如 XIVa和 XVIII）在两组中表现出不同的丰度模式，表明肠道微生物群平衡存在潜在差异。总之，CDI阳性患者及抗生素治疗后XI增加而 减少，这表明 比值的变化可能是一种有前景的生物标志物，值得进一步研究用于CDI诊断。
