Yuan Yue, Niu Xin, Hao Chenguang, Liang Lingyue, Huang Zubin, Wang Dongmei, Hong Meiling, Ding Li
Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, Ministry of Education, Key Laboratory for Ecology of Tropical Islands, College of Life Sciences, Hainan Normal University, Haikou 571158, China.
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571158, China.
Int J Mol Sci. 2025 Jan 7;26(2):445. doi: 10.3390/ijms26020445.
Taurine is essential for sustaining the body's physiological equilibrium and is extensively utilized as a dietary supplement and immune system enhancer for aquatic creatures. The gut microbiota serves as a vital health indicator in animals. In this study, our goal was to evaluate the effects of dietary taurine on the gut microbiome of Chinese stripe-necked turtles (). Turtles were evenly divided into three supplementation groups: a control group with no taurine in the diet, a low group with 0.1% taurine, and a high group with 0.4% taurine. High-throughput sequencing was employed to analyze the gut microbiome's composition and structure. The results showed that the impact of taurine supplementation on the α-diversity and β-diversity of the gut microbiome was not statistically significant. At the phylum level, Firmicutes and Bacteroidetes predominated the gut microbiome. At the genus level, three beneficial bacteria, , , and showed significant differences under different levels of taurine supplementation and increased in abundance with the supplementation of taurine, while the harmful bacterium decreased in abundance with the addition of taurine and exhibited significant differences. Furthermore, LEfSe analysis and functional predictions highlighted significant variations in the functional traits of gram-positive intestinal bacteria among the different taurine supplementation levels. These findings imply that dietary taurine may alter the intestinal microbiome structure in Chinese stripe-necked turtles, offering valuable insights for the scientific assessment of taurine's health benefits as a feed additive and potentially guiding the formulation of more informed and healthier feeding practices.
牛磺酸对于维持身体的生理平衡至关重要，并且被广泛用作水生生物的膳食补充剂和免疫系统增强剂。肠道微生物群是动物重要的健康指标。在本研究中，我们的目标是评估日粮中牛磺酸对中华花龟肠道微生物群的影响。将龟平均分为三个补充组：日粮中不添加牛磺酸的对照组、添加0.1%牛磺酸的低剂量组和添加0.4%牛磺酸的高剂量组。采用高通量测序分析肠道微生物群的组成和结构。结果表明，补充牛磺酸对肠道微生物群的α多样性和β多样性的影响无统计学意义。在门水平上，厚壁菌门和拟杆菌门在肠道微生物群中占主导地位。在属水平上，三种有益菌，[具体菌名缺失]、[具体菌名缺失]和[具体菌名缺失]在不同牛磺酸补充水平下表现出显著差异，且随着牛磺酸的补充丰度增加，而有害菌[具体菌名缺失]随着牛磺酸的添加丰度降低并表现出显著差异。此外，LEfSe分析和功能预测突出了不同牛磺酸补充水平下革兰氏阳性肠道细菌功能特征的显著差异。这些发现表明，日粮中的牛磺酸可能会改变中华花龟的肠道微生物群结构，为科学评估牛磺酸作为饲料添加剂的健康益处提供有价值的见解，并可能指导制定更合理、更健康的喂养方式。
