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盐胁迫下枸杞中-()基因家族的全基因组鉴定及的潜在功能

Genome-Wide Identification of - () Gene Family and the Potential Function of Under Salt Stress in .

作者信息

Zhao Yizhu, Xu Jiahao, Xu Xinyi, Liu Hui, Chang Qinxiang, Xu Ling, Liang Zongsuo

机构信息

Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China.

The UWA Institute of Agriculture, UWA School of Agriculture and Environment, Faculty of Science, The University of Western Australia, Crawley, WA 6009, Australia.

出版信息

Int J Mol Sci. 2025 Jan 16;26(2):724. doi: 10.3390/ijms26020724.

Abstract

is an important medicinal herb known as a "natural antibiotic", which has been used in Southeast Asia for thousands of years. The () gene is an important regulatory factor for plant photoperiod flowering and stress response. However, there is currently no detailed research on the genes of . In our study, we performed a genome-wide analysis of () family members using bioinformatics tools and identified nine genes. Based on phylogenetic analysis, s were categorized into three groups, with members of the same group having similar structures. Gene duplication events indicated that only one pair of duplicated genes was identified, possibly caused by segmental duplication. In terms of evolutionary relationships, the COL proteins of and were closely related, showing that they are highly similar in the phylogenetic tree. In addition, genes showed tissue specificity and were specifically highly expressed mainly in leaves and flowers. Based on the cis-regulatory element prediction results, we examined the expression levels of s under hormone and salt stress, and was significantly induced. With subcellular localization results consistent with the prediction, we transformed into yeast and showed significant resistance to salt stress. Our study suggests that genes have important roles in response to abiotic stress and plant development and initially identifies key genes for future molecular regulation studies.

摘要

是一种重要的药用植物,被誉为“天然抗生素”,在东南亚已使用了数千年。()基因是植物光周期开花和胁迫响应的重要调控因子。然而,目前关于的基因尚无详细研究。在我们的研究中,我们使用生物信息学工具对()家族成员进行了全基因组分析,并鉴定出9个基因。基于系统发育分析,被分为三组,同一组的成员具有相似的结构。基因复制事件表明仅鉴定出一对复制基因,可能是由片段复制引起的。在进化关系方面,和的COL蛋白密切相关,表明它们在系统发育树中高度相似。此外,基因表现出组织特异性,主要在叶片和花朵中特异性高表达。基于顺式调控元件预测结果,我们检测了在激素和盐胁迫下的表达水平,并且被显著诱导。由于亚细胞定位结果与预测一致,我们将转入酵母并显示出对盐胁迫的显著抗性。我们的研究表明,基因在响应非生物胁迫和植物发育中具有重要作用,并初步鉴定出未来分子调控研究的关键基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f879/11765704/5a3b425739e6/ijms-26-00724-g001.jpg

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