Nakamura Maki, Oyane Ayako, Inose Tomoya, Kanemoto Yukimi, Miyaji Hirofumi
Nanomaterials Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), AIST Tsukuba Central 5, 1-1-1 Higashi, Tsukuba 305-8565, Japan.
Periodontics, Hokkaido University Hospital, N14W5, Kita-ku, Sapporo 060-8648, Japan.
Int J Mol Sci. 2025 Jan 20;26(2):852. doi: 10.3390/ijms26020852.
Lactoferrin is a highly safe antibacterial protein found in the human body and in foods. Calcium phosphate (CaP) nanoparticles with immobilized lactoferrin could therefore be useful as intraoral disinfectants for the prevention and treatment of dental infections because CaP is a mineral component of human teeth. In this study, we fabricated CaP nanoparticles with co-immobilized lactoferrin and heparin using a simple one-step coprecipitation process. Heparin, a negatively charged polysaccharide, was used as both an immobilizing agent for lactoferrin and a particle-dispersing agent. The immobilization efficiency for lactoferrin in the CaP nanoparticles depended on the concentrations of both the lactoferrin and heparin in the reaction solution and was over 90% under optimal conditions. The nanoparticles had a hydrodynamic diameter of about 150-200 nm and could be well dispersed in water, owing to their relatively large negative zeta potential derived from heparin. They were found to exhibit antibacterial activity against , which is involved in the initial formation of dental plaque that consequently leads to dental caries and periodontal disease. These results indicate the potential of the proposed nanoparticles as intraoral disinfectants.
乳铁蛋白是一种在人体和食物中发现的高度安全的抗菌蛋白。因此，固定有乳铁蛋白的磷酸钙（CaP）纳米颗粒可用作口腔内消毒剂，用于预防和治疗牙齿感染，因为CaP是人类牙齿的矿物质成分。在本研究中，我们使用简单的一步共沉淀法制备了共固定有乳铁蛋白和肝素的CaP纳米颗粒。肝素是一种带负电荷的多糖，既用作乳铁蛋白的固定剂，又用作颗粒分散剂。CaP纳米颗粒中乳铁蛋白的固定效率取决于反应溶液中乳铁蛋白和肝素的浓度，在最佳条件下超过90%。纳米颗粒的流体动力学直径约为150-200nm，由于其源自肝素的相对较大的负zeta电位，能够很好地分散在水中。它们被发现对 具有抗菌活性， 参与牙菌斑的初始形成，进而导致龋齿和牙周疾病。这些结果表明了所提出的纳米颗粒作为口腔内消毒剂的潜力。
