Laoung-On Jiraporn, Nuchniyom Pimchanok, Intui Ketsarin, Jaikang Churdsak, Saenphet Kanokporn, Boonyapranai Kongsak, Konguthaithip Giatgong, Outaitaveep Nopparuj, Phankhieo Sasitorn, Sudwan Paiwan
Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Office of Research Administration, Chiang Mai University, Chiang Mai 50200, Thailand.
Life (Basel). 2024 Dec 24;15(1):6. doi: 10.3390/life15010006.
Mancozeb (MZ), an EBDC fungicide, has been found to contaminate agricultural products and cause male reproductive toxicity. The phytochemical compounds of white petal extract (WNPE) and its effects on metabolomic profiles and reproductive function in male rats poisoned with MZ were investigated. Seventy-two mature male Wistar rats were divided into nine groups ( = 8) and, for 30 days, were gavaged with WNPE at doses of 0.55, 1.10, and 2.20 mg/kg; were given distilled water; or were co-gavaged with MZ and WNPE. By evaluating the H-NMR of WNPE, myricetin, apigenin, luteolin, ferulic acid, caffeic acid, ascorbic acid, genistein, chlorogenic acid, naringenin, and ellagic acid were found, and the essential minerals were evaluated by AAS. The NMR spectra demonstrated that creatine, carnitine, ACh, and choline in WNPE were significantly higher than that in MZ. The gavaging of the rats with WNPE before poisoning them with MZ improved creatine, carnitine, acetylcholine, progressive sperm motility, sperm viability, and normal sperm morphology compared to rats who only received MZ. It was concluded that MZ had a toxicity effect on the male reproductive system via decreased metabolomic profiles, affecting sperm motility, sperm viability, and normal sperm morphology. Nevertheless, WNPE had plenty of bioactive compounds that could enhance creatine, carnitine, and acetylcholine, which are related to sperm quality in male rats. WNPE should be considered as an alternative dietary supplement that can protect against MZ toxicity and enhance sperm quality in the male rat reproductive system.
代森锰锌（MZ）是一种乙撑双二硫代氨基甲酸盐类杀菌剂，已被发现会污染农产品并导致雄性生殖毒性。本研究调查了白花瓣提取物（WNPE）的植物化学成分及其对MZ中毒雄性大鼠代谢组学特征和生殖功能的影响。将72只成熟雄性Wistar大鼠分为9组（每组 = 8只），连续30天分别以0.55、1.10和2.20 mg/kg的剂量灌胃WNPE；给予蒸馏水；或同时灌胃MZ和WNPE。通过评估WNPE的氢核磁共振（H-NMR），发现了杨梅素、芹菜素、木犀草素、阿魏酸、咖啡酸、抗坏血酸、染料木黄酮、绿原酸、柚皮素和鞣花酸，并采用原子吸收光谱法（AAS）评估了必需矿物质。核磁共振光谱表明，WNPE中的肌酸、肉碱、乙酰胆碱和胆碱显著高于MZ中的含量。与仅接受MZ的大鼠相比，在给大鼠用MZ中毒前灌胃WNPE可改善肌酸、肉碱、乙酰胆碱、精子前向运动能力、精子活力和正常精子形态。研究得出结论，MZ通过降低代谢组学特征对雄性生殖系统产生毒性作用，影响精子运动能力、精子活力和正常精子形态。然而，WNPE含有大量生物活性化合物，可增强与雄性大鼠精子质量相关的肌酸、肉碱和乙酰胆碱。WNPE应被视为一种替代性膳食补充剂，可预防MZ毒性并提高雄性大鼠生殖系统的精子质量。
