Banti Matteo, Garcia-Gil Mercedes, Guidotti Lorenzo, Di Giuseppe Graziano, Rapposelli Simona, Monti Daniela, Tampucci Silvia, De Leo Marinella, Gado Francesca, Nieri Paola, Manera Clementina
Department of Pharmacy, University of Pisa, Via Bonanno, 6, 56126 Pisa, Italy.
Department of Biology, University of Pisa, Via Luca Ghini, 13, 56126 Pisa, Italy.
Molecules. 2025 Jan 8;30(2):224. doi: 10.3390/molecules30020224.
Hearing loss is one of the most common sensory disorders in humans, and a large number of cases are due to ear cell damage caused by ototoxic drugs including anticancer agents, such as cisplatin. The recent literature reported that hearing loss is promoted by an excessive generation of reactive oxygen species (ROS) in cochlea cells, which causes oxidative stress. Recently, polysaccharides from the cyanobacterium showed many biological activities, including antioxidant activity, suggesting their potential use to combat hearing loss. On these bases, this study describes the extraction, purification, and characterization of water-soluble polysaccharides from (SPPs) and the investigation of their protective role against cisplatin toxicity on House Ear Institute-Organ of Corti (HEI-OC1) cells. The results showed that SPPs (5-80 µg/mL) induced a dose-dependent increase in viability, statistically significant at 40 µg/mL and 80 µg/mL. Moreover, SPPs, evaluated at 80 µg/mL, inhibited the cisplatin-induced ROS level increase in HEI-OC1. This evidence highlights the potential of SPPs as natural candidates to protect cochlear ear cells against ototoxic oxidative agents. Moreover, in view of the potential use of microalgal polysaccharides to realize hydrogels, SPPs could also represent a healthy carrier for other topically administered otoprotective agents.
听力损失是人类最常见的感觉障碍之一，大量病例是由包括顺铂等抗癌药物在内的耳毒性药物引起的耳细胞损伤所致。最近的文献报道，耳蜗细胞中活性氧（ROS）的过度产生会促进听力损失，这会导致氧化应激。最近，来自蓝藻的多糖显示出许多生物活性，包括抗氧化活性，表明它们在对抗听力损失方面具有潜在用途。基于这些，本研究描述了从[蓝藻名称未给出]中提取、纯化和表征水溶性多糖（SPPs），并研究了它们对顺铂对耳科研究所-柯蒂氏器（HEI-OC1）细胞毒性的保护作用。结果表明，SPPs（5-80μg/mL）诱导细胞活力呈剂量依赖性增加，在40μg/mL和80μg/mL时具有统计学意义。此外，在80μg/mL下评估的SPPs抑制了顺铂诱导的HEI-OC1中ROS水平的升高。这一证据突出了SPPs作为保护耳蜗耳细胞免受耳毒性氧化剂侵害的天然候选物的潜力。此外，鉴于微藻多糖在制备水凝胶方面的潜在用途，SPPs也可能成为其他局部给药耳保护剂的健康载体。
