Hueso P, Reglero A, Rodrigo M, Cabezas J A, Reuter G, Schauer R
Biol Chem Hoppe Seyler. 1985 Feb;366(2):167-71. doi: 10.1515/bchm3.1985.366.1.167.
Two major gangliosides from pig spleen lymphocytes, accounting for 57% of the total lipid-bound sialic acids, were isolated and purified to homogeneity by column chromatography on DEAE-Sephadex and silica gel. They were identified as GM3 (II3Neu5GcLacCer), and GD3 (II3(Neu5Gc)2LacCer), by thin-layer chromatography in comparison with standards and by analysis of the constituent sugars. The major fatty acids of these gangliosides were stearic acid and myristic acid, respectively. In addition to these gangliosides, GD2 and bands comigrating on thin-layer chromatography with authentic GM2, GM1, GD1a and GD1b were found. These compounds also occur in pig peripheral blood lymphocytes, where, however, GD3 represents about 70% of the total lipid-bound sialic acid.
从猪脾脏淋巴细胞中分离出两种主要神经节苷脂，它们占总脂质结合唾液酸的57%，通过DEAE-葡聚糖凝胶柱色谱法和硅胶柱色谱法进行分离纯化，直至达到均一状态。通过与标准品进行薄层色谱比较以及对组成糖进行分析，将它们鉴定为GM3（II3Neu5GcLacCer）和GD3（II3(Neu5Gc)2LacCer）。这些神经节苷脂的主要脂肪酸分别是硬脂酸和肉豆蔻酸。除了这些神经节苷脂外，还发现了GD2以及在薄层色谱上与正品GM2、GM1、GD1a和GD1b迁移率相同的条带。这些化合物也存在于猪外周血淋巴细胞中，然而，在猪外周血淋巴细胞中，GD3约占总脂质结合唾液酸的70%。
