小豆蔻和茴香对HO诱导的SH-SY5Y细胞氧化应激及无细胞试验的多价神经保护活性

Multivalent Neuroprotective Activity of (Cardamom) and (Fennel) in HO-Induced Oxidative Stress in SH-SY5Y Cells and Acellular Assays.

作者信息

Sharma Himadri, Yang Hyewon, Sharma Niti, An Seong Soo A

机构信息

Department of Bionano Technology, Gachon Bionano Research Institute, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si 461-701, Gyeonggi-do, Republic of Korea.

出版信息

Pharmaceuticals (Basel). 2024 Dec 24;18(1):2. doi: 10.3390/ph18010002.

DOI:10.3390/ph18010002

PMID:39861065

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11768314/

Abstract

BACKGROUND

(Cardamom) and (Fennel) are well-known spices and are also used as natural mouth fresheners. This study was performed to evaluate their neuroprotective ability based on certain acellular and cellular assays.

METHODS

Hexane and ethyl acetate extracts were prepared using cardamom and fennel seeds. GC/MS was performed for the identification of important bioactive compounds. Cell-based assays were performed using SH-SY5Y cells. Hydrogen peroxide was used for the induction of oxidative stress, and evaluation was done based on neuroprotection, reduced reactive oxygen species, and restoration of mitochondrial membrane potential (MMP). Additionally, anti-Aβ fibrillization/oligomerization activities were also analyzed along with anti-acetylcholinesterase activity.

RESULTS

α-Terpinyl acetate and anethol were identified as major phytocompounds in cardamom and fennel, respectively. Cardamom extracts and α-terpinyl acetate were more potent acetylcholinesterase (AChE) inhibitors than fennel extracts and anethol [IC cardamom extracts, 130-150 μg/mL; α-terpinyl acetate, 61.87 μg/mL; anethol, 374.2 μg/mL; fennel extracts, >1 mg/mL] and showed mixed-type inhibition. Only the extracts displayed potent anti-Aβ fibrilization activity (>50%). Anethol showed potent anti-Aβ oligomerization activity (>50%), followed by α-terpinyl acetate and fennel-H (~36%). The neuroprotective potential of the spice extracts/phytochemicals was evaluated in SH-SY5Y cells by using HO-induced oxidative stress. Cardamom-EA displayed the best neuroprotection (0.01 to 30 μg/mL). No neuroprotection was observed by α-terpinyl acetate and anethol. Cardamom extracts and fennel-H restored the normal reactive oxygen species (ROS) levels at 30 µg/mL and 1 µg/mL, respectively.

CONCLUSION

Overall, the extracts provided better neuroprotection than the pure compounds in cellular assays and displayed strong anti-Aβ fibrilization activity.

摘要

背景

小豆蔻和茴香是著名的香料，也用作天然口腔清新剂。本研究基于某些脱细胞和细胞试验来评估它们的神经保护能力。

方法

用小豆蔻籽和茴香籽制备己烷和乙酸乙酯提取物。采用气相色谱-质谱联用（GC/MS）鉴定重要的生物活性化合物。使用SH-SY5Y细胞进行基于细胞的试验。用过氧化氢诱导氧化应激，并基于神经保护、活性氧减少和线粒体膜电位（MMP）恢复进行评估。此外，还分析了抗Aβ纤维化/寡聚化活性以及抗乙酰胆碱酯酶活性。

结果

乙酸松油酯和茴香脑分别被鉴定为小豆蔻和茴香中的主要植物化合物。小豆蔻提取物和乙酸松油酯比茴香提取物和茴香脑更有效地抑制乙酰胆碱酯酶（AChE）[小豆蔻提取物的IC50，130 - 150μg/mL；乙酸松油酯，61.87μg/mL；茴香脑，374.2μg/mL；茴香提取物，>1mg/mL]，并表现出混合型抑制。只有提取物显示出强大的抗Aβ纤维化活性（>50%）。茴香脑显示出强大的抗Aβ寡聚化活性（>50%），其次是乙酸松油酯和茴香-H（~36%）。通过使用过氧化氢诱导的氧化应激在SH-SY5Y细胞中评估香料提取物/植物化学物质的神经保护潜力。小豆蔻-乙酸乙酯显示出最佳的神经保护作用（0.01至30μg/mL）。乙酸松油酯和茴香脑未观察到神经保护作用。小豆蔻提取物和茴香-H分别在30μg/mL和1μg/mL时恢复了正常的活性氧（ROS）水平。