Department of Pathology, Cangzhou Central Hospital, Changzhou, China.
Department of Orthopaedics, Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine of Hebei Province, Cangzhou, China.
Thorac Cancer. 2019 Oct;10(10):1953-1961. doi: 10.1111/1759-7714.13174. Epub 2019 Aug 26.
To evaluate the EZR (ezrin) gene expression in breast cancer and correlation with the prognosis through bioinformatics analysis and immunohistochemistry assay.
EZR gene expression in breast cancer and corresponding normal breast tissue was compared in the TCGA database. Protein-protein interaction (PPI) network relevant EZR was established through the STRING database. The correlation between EZR expression and prognosis of breast cancer was analyzed by the log-rank analysis from the TCGA. Ezrin protein (coded by EZR) expression was also examined by immunohistochemistry assay in 120 breast cancer patients.
EZR expression level in tumor tissue was significantly upregulated compared to that of normal breast tissue of breast cancer patients (P < 0.05). In the PPI analysis, there were 51 nodes and 455 edges in the network. The top 10 hub genes of the network were identified. High expression of EZR mRNA was correlated with poor overall survival (OS) of the breast cancer patients (HR = 1.40, P = 0.038). However, the disease-free survival (DFS) of breast cancer patients did not correlate with the EZR mRNA level (HR = 0.86, P = 0.44). The ezrin protein expression was positive with uniform brown-yellow granules in the cell membrane, cavity surface and cytoplasm of the breast cancer cells. Of the included 120 cancer samples, 98 cases were positive for ezrin expression and 22 were negative. No correlation was found between ezrin expression site and patients' clinicopathological features.
EZR is upregulated in breast cancer and can be used as potential biomarker for overall survival.
通过生物信息学分析和免疫组织化学检测，评估乳腺癌中 EZR（埃兹蛋白）基因的表达，并探讨其与预后的相关性。
在 TCGA 数据库中比较乳腺癌和相应正常乳腺组织中 EZR 基因的表达。通过 STRING 数据库构建与 EZR 相关的蛋白质-蛋白质相互作用（PPI）网络。通过 TCGA 的对数秩分析分析 EZR 表达与乳腺癌预后的相关性。通过免疫组织化学检测 120 例乳腺癌患者的 Ezrin 蛋白（由 EZR 编码）表达。
与乳腺癌患者的正常乳腺组织相比，肿瘤组织中 EZR 表达水平明显上调（P<0.05）。在 PPI 分析中，网络中有 51 个节点和 455 个边。确定了网络的前 10 个枢纽基因。EZR mRNA 高表达与乳腺癌患者的总生存期（OS）不良相关（HR=1.40，P=0.038）。然而，乳腺癌患者的无病生存期（DFS）与 EZR mRNA 水平无关（HR=0.86，P=0.44）。Ezrin 蛋白表达阳性，乳腺癌细胞的细胞膜、腔面和细胞质呈现均匀的棕黄色颗粒。在纳入的 120 例癌症样本中，98 例 Ezrin 表达阳性，22 例阴性。Ezrin 表达部位与患者的临床病理特征之间无相关性。
EZR 在乳腺癌中上调，可作为总生存期的潜在生物标志物。
