Hong Soyeon, Lee Hee Ju, Jung Da Seul, Erdenebileg Saruul, Hwang Hoseong, Kwon Hak Cheol, Kwon Jaeyoung, Yoo Gyhye
Smart Farm Research Center, Korean Institute of Science and Technology (KIST), Gangneung 25451, Republic of Korea.
Center for Natural Product Systems Biology, Korean Institute of Science and Technology, Gangneung 25451, Republic of Korea.
Pharmaceuticals (Basel). 2025 Jan 3;18(1):45. doi: 10.3390/ph18010045.
Osteoporosis is characterized by the microstructural depletion of bone tissue and decreased bone density, leading to an increased risk of fractures. Nakai, an endemic species of the Korean Peninsula, grows wild in Ulleungdo. In this study, we aimed to investigate the effects of and its components on osteoporosis.
The alkaline phosphatase (ALP) activity of extracts and fractions was evaluated in MC3T3-E1 pre-osteoblasts, and the -hexane fraction (CWH) showed the best properties for ALP activity. The effects of the CWH on bone formation were assessed in MC3T3-E1 cells and ovariectomized mice. Biochemical assays and histological analyses focused on the signaling activation of osteoblast differentiation and osteogenic markers, such as ALP, collagen, and osterix. The CWH significantly activated TGF-β and Wnt signaling, enhancing osteoblast differentiation and bone matrix formation. Notably, CWH treatment improved micro-CT indices, such as femoral bone density, and restored serum osteocalcin levels compared to OVX controls.
These results highlight the potential of the Nakai -hexane fraction as a promising therapeutic agent for managing osteoporosis.
骨质疏松症的特征是骨组织微观结构损耗和骨密度降低，导致骨折风险增加。中井（Nakai）是朝鲜半岛的特有物种，在郁陵岛野生生长。在本研究中，我们旨在研究中井及其成分对骨质疏松症的影响。
在MC3T3-E1前成骨细胞中评估了中井提取物和馏分的碱性磷酸酶（ALP）活性，其中正己烷馏分（CWH）显示出最佳的ALP活性特性。在MC3T3-E1细胞和去卵巢小鼠中评估了CWH对骨形成的影响。生化分析和组织学分析重点关注成骨细胞分化和成骨标志物（如ALP、胶原蛋白和osterix）的信号激活。CWH显著激活TGF-β和Wnt信号，增强成骨细胞分化和骨基质形成。值得注意的是，与去卵巢对照组相比，CWH治疗改善了微观CT指标，如股骨骨密度，并恢复了血清骨钙素水平。
这些结果突出了中井正己烷馏分作为治疗骨质疏松症的有前景治疗剂的潜力。
