Wang Haisu, Yuan Xiaolong, Huang Xinrong, Zhang Peng, Gu Gan
Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China.
Plants (Basel). 2025 Jan 9;14(2):173. doi: 10.3390/plants14020173.
Endophytic fungi possess a unique ability to produce abundant secondary metabolites, which play an active role in the growth and development of host plants. In this study, chemical investigations on the endophytic fungus TE-739D derived from the cultivated tobacco ( L.) afforded two new polyketide derivatives, namely japoniones A () and B (), as well as four previously reported compounds -. Their chemical structures were elucidated by detailed spectroscopic analyses and quantum chemical calculations. In the herbicidal assays on the germination and radicle growth of L. and seeds, compound was found to inhibit the germ and radicle elongation. Notably, compound showed potent herbicidal activity against L. germ elongation, with an IC value of 43.6 μg/mL, even higher than the positive control glyphosate (IC = 76.0 μg/mL). Moreover, compound demonstrated strong antibacterial effects against the pathogens and , with a comparable MIC value of 16 μg/mL to the positive control chloramphenicol. These findings indicate that the endophytic fungus TE-739D holds significant metabolic potential to produce bioactive secondary metabolites, which are beneficial, providing survival value to the host plants.
内生真菌具有产生丰富次生代谢产物的独特能力，这些次生代谢产物在宿主植物的生长发育中发挥着积极作用。在本研究中，对源自栽培烟草（Nicotiana tabacum L.）的内生真菌TE-739D进行化学研究，得到了两种新的聚酮衍生物，即日本菌素A（japoniones A）和B（japoniones B），以及四种先前报道的化合物 - 。通过详细的光谱分析和量子化学计算阐明了它们的化学结构。在对烟草（Nicotiana tabacum L.）和拟南芥（Arabidopsis thaliana）种子萌发和胚根生长的除草活性测定中，发现化合物 抑制了胚和胚根的伸长。值得注意的是，化合物 对烟草（Nicotiana tabacum L.）胚伸长表现出强效除草活性，IC50值为43.6 μg/mL，甚至高于阳性对照草甘膦（IC50 = 76.0 μg/mL）。此外，化合物 对病原菌枯草芽孢杆菌（Bacillus subtilis）和大肠杆菌（Escherichia coli）表现出强烈的抗菌作用，MIC值为16 μg/mL，与阳性对照氯霉素相当。这些发现表明，内生真菌TE-739D具有产生生物活性次生代谢产物的显著代谢潜力，这些次生代谢产物对宿主植物有益，具有生存价值。
