Sharma Amit Raj, Gajurel Gaurav, Abdel-Karim Salma, Alam Mohammad Abrar, Shields Robert Colquhoun, Medina-Bolivar Fabricio
Arkansas Biosciences Institute, Arkansas State University, Jonesboro, AR 72401, USA.
Molecular Biosciences Graduate Program, Arkansas State University, Jonesboro, AR 72401, USA.
Plants (Basel). 2025 Jan 17;14(2):259. doi: 10.3390/plants14020259.
Phenolic compounds, such as stilbenes and flavonoids, from spp. exhibit diverse biological activities, including antimicrobial, anti-inflammatory, and cytotoxicity properties. To this end, the objectives of this study were to establish hairy root cultures of and assess its capacity to produce these bioactive compounds. The co-treatment of the hairy root cultures with the elicitors methyl-β-cyclodextrin, magnesium chloride, methyl jasmonate, and hydrogen peroxide led to the production and secretion of the geranylated flavanone malheuran A, which was confirmed by mass spectrometry and NMR spectroscopy analyses. The highest amount (104.3 ± 6.9 mg/L) of malheuran A was detected at 192 h after elicitor treatment. The elicited hairy roots were re-elicited for 192 h with the same combination of elicitors, and they produced a substantial amount of malheuran A (91.9 ± 6.8 mg/L). Malheuran A, purified from extracts of the hairy root culture medium, showed potent antimicrobial activity against Gram-positive bacteria, including methicillin-resistant and vancomycin-resistant Enterococci. It also demonstrated anti-inflammatory effects by suppressing nitrite production in LPS-stimulated RAW 264.7 macrophages. These findings show that various elicitor treatments can boost malheuran A production in hairy root cultures, making them a sustainable bioproduction platform for this bioactive specialized metabolite.
来自[物种名称]的酚类化合物，如芪类和黄酮类化合物，具有多种生物活性，包括抗菌、抗炎和细胞毒性。为此，本研究的目的是建立[物种名称]的毛状根培养物，并评估其产生这些生物活性化合物的能力。用引发剂甲基-β-环糊精、氯化镁、茉莉酸甲酯和过氧化氢对毛状根培养物进行联合处理，导致香叶基化黄烷酮麦芽花素A的产生和分泌，这通过质谱和核磁共振光谱分析得到证实。在引发剂处理后192小时检测到麦芽花素A的最高含量（104.3±6.9毫克/升）。用相同的引发剂组合对诱导的毛状根再诱导192小时，它们产生了大量的麦芽花素A（91.9±6.8毫克/升）。从毛状根培养基提取物中纯化的麦芽花素A对革兰氏阳性菌表现出强大的抗菌活性，包括耐甲氧西林金黄色葡萄球菌和耐万古霉素肠球菌。它还通过抑制脂多糖刺激的RAW 264.7巨噬细胞中亚硝酸盐的产生表现出抗炎作用。这些发现表明，各种引发剂处理可以提高毛状根培养物中麦芽花素A的产量，使其成为这种生物活性特殊代谢产物的可持续生物生产平台。
