• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过整合网络药理学分析与实验验证对L.提取物抗结直肠癌抗癌机制的研究

An Investigation of the Anticancer Mechanism of L. Extract Against Colorectal Cancer by Integrating a Network Pharmacological Analysis and Experimental Validation.

作者信息

Jeong Mibae, Chun Jaemoo, Park Sang-Min, Yeo Heerim, Na Se Won, Ha In Jin, Kim Bonglee, Jeong Mi-Kyung

机构信息

KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Republic of Korea.

KIOM School, University of Science & Technology (UST), Daejeon 34054, Republic of Korea.

出版信息

Plants (Basel). 2025 Jan 18;14(2):263. doi: 10.3390/plants14020263.

DOI:10.3390/plants14020263
PMID:39861616
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11768342/
Abstract

L. has exhibited various pharmacological effects, yet its anticancer activities against colorectal cancer (CRC) and underlying molecular mechanisms remain unclear. This study investigated the anticancer properties of an ethanol extract of L. (CSE) against CRC cells, focusing on the identification of bioactive compounds and their mechanisms of action. A network pharmacology analysis was conducted to identify potential CRC targets and bioactive compounds of CSE, using LC-MS for compound identification. The anticancer effects of CSE were then validated through in vitro and in vivo models of CRC. The network pharmacological approach identified 87 overlapping genes between CSE targets and CRC-related genes, with protein-protein interaction analysis highlighting 33 key target genes. CSE inhibited cell proliferation in human CRC cell lines, including HCT 116, KM12SM, HT-29, and COLO 205, and induced apoptosis via caspase 3/7 activation. Western blot analyses confirmed the modulation of critical signaling pathways, including STAT3, AKT, and mitogen-activated protein kinases. Furthermore, CSE significantly suppressed tumor growth in MC38 CRC-bearing mice. These findings suggest that CSE possesses substantial potential as a natural anticancer agent for CRC treatment, highlighting the need for further exploration in therapeutic development.

摘要

L.已表现出多种药理作用，但其对结直肠癌（CRC）的抗癌活性及潜在分子机制仍不清楚。本研究调查了L.乙醇提取物（CSE）对CRC细胞的抗癌特性，重点是生物活性化合物的鉴定及其作用机制。进行了网络药理学分析以鉴定CSE的潜在CRC靶点和生物活性化合物，并使用液相色谱-质谱联用（LC-MS）进行化合物鉴定。然后通过CRC的体外和体内模型验证了CSE的抗癌作用。网络药理学方法确定了CSE靶点与CRC相关基因之间的87个重叠基因，蛋白质-蛋白质相互作用分析突出了33个关键靶基因。CSE抑制人CRC细胞系（包括HCT 116、KM12SM、HT-29和COLO 205）的细胞增殖，并通过激活半胱天冬酶3/7诱导细胞凋亡。蛋白质印迹分析证实了关键信号通路（包括信号转导和转录激活因子3（STAT3）、蛋白激酶B（AKT）和丝裂原活化蛋白激酶）的调节。此外，CSE显著抑制MC38荷CRC小鼠的肿瘤生长。这些发现表明，CSE作为一种用于CRC治疗的天然抗癌剂具有巨大潜力，突出了在治疗开发中进一步探索的必要性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e5b/11768342/6059ad031418/plants-14-00263-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e5b/11768342/e5af226cee6f/plants-14-00263-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e5b/11768342/67f88985a949/plants-14-00263-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e5b/11768342/fc01db346f81/plants-14-00263-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e5b/11768342/92c80c982549/plants-14-00263-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e5b/11768342/ad6cf87df106/plants-14-00263-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e5b/11768342/da7f7fa65c16/plants-14-00263-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e5b/11768342/f24a4ffc11ba/plants-14-00263-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e5b/11768342/dae43238c946/plants-14-00263-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e5b/11768342/d1c8b12f1e0f/plants-14-00263-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e5b/11768342/6059ad031418/plants-14-00263-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e5b/11768342/e5af226cee6f/plants-14-00263-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e5b/11768342/67f88985a949/plants-14-00263-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e5b/11768342/fc01db346f81/plants-14-00263-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e5b/11768342/92c80c982549/plants-14-00263-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e5b/11768342/ad6cf87df106/plants-14-00263-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e5b/11768342/da7f7fa65c16/plants-14-00263-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e5b/11768342/f24a4ffc11ba/plants-14-00263-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e5b/11768342/dae43238c946/plants-14-00263-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e5b/11768342/d1c8b12f1e0f/plants-14-00263-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e5b/11768342/6059ad031418/plants-14-00263-g010.jpg

相似文献

1
An Investigation of the Anticancer Mechanism of L. Extract Against Colorectal Cancer by Integrating a Network Pharmacological Analysis and Experimental Validation.通过整合网络药理学分析与实验验证对L.提取物抗结直肠癌抗癌机制的研究
Plants (Basel). 2025 Jan 18;14(2):263. doi: 10.3390/plants14020263.
2
A network pharmacology approach and experimental validation to investigate the anticancer mechanism and potential active targets of ethanol extract of Wei-Tong-Xin against colorectal cancer through induction of apoptosis via PI3K/AKT signaling pathway.基于网络药理学方法和实验验证，探讨味通心通过诱导 PI3K/AKT 信号通路细胞凋亡对结肠癌的抗癌机制和潜在的活性靶点。
J Ethnopharmacol. 2023 Mar 1;303:115933. doi: 10.1016/j.jep.2022.115933. Epub 2022 Nov 18.
3
Mechanism of Bazhen decoction in the treatment of colorectal cancer based on network pharmacology, molecular docking, and experimental validation.基于网络药理学、分子对接和实验验证的八珍汤治疗大肠癌的作用机制。
Front Immunol. 2023 Sep 20;14:1235575. doi: 10.3389/fimmu.2023.1235575. eCollection 2023.
4
Anti-inflammatory activity of an ethanolic Caesalpinia sappan extract in human chondrocytes and macrophages.没食子酸巴西苏木素乙醇提取物对人软骨细胞和巨噬细胞的抗炎活性。
J Ethnopharmacol. 2011 Nov 18;138(2):364-72. doi: 10.1016/j.jep.2011.09.011. Epub 2011 Sep 21.
5
Anticancer activity of by downregulating mitochondrial genes in A549 lung cancer cell line.通过下调 A549 肺癌细胞中线粒体基因的表达发挥抗癌活性。
F1000Res. 2022 Feb 11;11:169. doi: 10.12688/f1000research.76187.2. eCollection 2022.
6
Integrated network pharmacology and experimental verification to investigate the mechanisms of YYFZBJS against colorectal cancer CDK1/PI3K/Akt signaling.综合网络药理学与实验验证以探究益元扶正散结方抗结直肠癌CDK1/PI3K/Akt信号传导的机制
Front Oncol. 2022 Nov 15;12:961653. doi: 10.3389/fonc.2022.961653. eCollection 2022.
7
Combining Network Pharmacology and Experimental Validation to Study the Action and Mechanism of Water extract of Asparagus Against Colorectal Cancer.结合网络药理学与实验验证研究芦笋水提取物抗结直肠癌的作用及机制
Front Pharmacol. 2022 Jun 14;13:862966. doi: 10.3389/fphar.2022.862966. eCollection 2022.
8
Understanding apoptotic induction by Sargentodoxa cuneata-Patrinia villosa herb pair via PI3K/AKT/mTOR signalling in colorectal cancer cells using network pharmacology and cellular studies.采用网络药理学和细胞研究方法探讨山乌龟-败酱草药对对结直肠癌细胞中 PI3K/AKT/mTOR 信号通路诱导细胞凋亡的作用机制。
J Ethnopharmacol. 2024 Jan 30;319(Pt 3):117342. doi: 10.1016/j.jep.2023.117342. Epub 2023 Oct 23.
9
Integrating UHPLC-Q-TOF-MS/MS, network pharmacology, bioinformatics and experimental validation to uncover the anti-cancer mechanisms of TiaoPi AnChang decoction in colorectal cancer.运用 UHPLC-Q-TOF-MS/MS、网络药理学、生物信息学和实验验证方法，揭示调脾安神汤治疗大肠癌的作用机制。
J Ethnopharmacol. 2024 Nov 15;334:118576. doi: 10.1016/j.jep.2024.118576. Epub 2024 Jul 14.
10
Inhibitory activities of Lignum Sappan extractives on growth and growth-related signaling of tumor cells.苏木提取物对肿瘤细胞生长及生长相关信号传导的抑制活性。
Chin J Nat Med. 2014 Aug;12(8):607-12. doi: 10.1016/S1875-5364(14)60092-3.

引用本文的文献

1
Phytochemical Characterization, Antioxidant Activity, and Anti-Melanoma Mechanism of Flower Buds of Pamp.南美蟛蜞菊花蕾的植物化学特征、抗氧化活性及抗黑色素瘤机制
Plants (Basel). 2025 Jun 5;14(11):1725. doi: 10.3390/plants14111725.

本文引用的文献

1
Network medicine and systems pharmacology approaches to predicting adverse drug effects.预测药物不良反应的网络医学和系统药理学方法。
Br J Pharmacol. 2024 Sep 11. doi: 10.1111/bph.17330.
2
Lyoniresinol attenuates cerebral ischemic stroke injury in MCAO rat based on oxidative stress suppression via regulation of Akt/GSK-3β/Nrf2 signaling.基于对Akt/GSK-3β/Nrf2信号通路的调节抑制氧化应激，来曲诺醇减轻大脑中动脉闭塞（MCAO）大鼠的脑缺血性中风损伤。
Biomed Pharmacother. 2023 Nov;167:115543. doi: 10.1016/j.biopha.2023.115543. Epub 2023 Sep 22.
3
Cancer chemoresistance and its mechanisms: Associated molecular factors and its regulatory role.
癌症化疗耐药性及其机制：相关分子因素及其调控作用。
Med Oncol. 2023 Aug 7;40(9):264. doi: 10.1007/s12032-023-02138-y.
4
Colorectal cancer and therapy response: a focus on the main mechanisms involved.结直肠癌与治疗反应：聚焦于相关主要机制
Front Oncol. 2023 Jul 19;13:1208140. doi: 10.3389/fonc.2023.1208140. eCollection 2023.
5
Mass spectrometry-based metabolomics for discovering active ingredients and exploring action mechanism of herbal medicine.基于质谱的代谢组学用于发现草药活性成分及探索其作用机制。
Front Chem. 2023 Mar 31;11:1142287. doi: 10.3389/fchem.2023.1142287. eCollection 2023.
6
Natural Products as Anticancer Agents: Current Status and Future Perspectives.天然产物作为抗癌剂：现状与展望。
Molecules. 2022 Nov 30;27(23):8367. doi: 10.3390/molecules27238367.
7
Linn. Ameliorates Allergic Nasal Inflammation by Upregulating the Keap1/Nrf2/HO-1 Pathway in an Allergic Rhinitis Mouse Model and Nasal Epithelial Cells.林恩。通过上调过敏性鼻炎小鼠模型和鼻上皮细胞中的Keap1/Nrf2/HO-1通路改善过敏性鼻炎症。
Antioxidants (Basel). 2022 Nov 15;11(11):2256. doi: 10.3390/antiox11112256.
8
Integrated network pharmacology analysis, molecular docking, LC-MS analysis and bioassays revealed the potential active ingredients and underlying mechanism of for COVID-19.综合网络药理学分析、分子对接、液相色谱-质谱联用分析和生物测定揭示了[药物名称]对2019冠状病毒病的潜在活性成分和潜在作用机制。 （原文中“for COVID-19”前缺少具体药物名称，翻译时补充为“[药物名称]对2019冠状病毒病” ）
Front Plant Sci. 2022 Sep 15;13:988655. doi: 10.3389/fpls.2022.988655. eCollection 2022.
9
Anticancer activity of by downregulating mitochondrial genes in A549 lung cancer cell line.通过下调 A549 肺癌细胞中线粒体基因的表达发挥抗癌活性。
F1000Res. 2022 Feb 11;11:169. doi: 10.12688/f1000research.76187.2. eCollection 2022.
10
Multi-compound and drug-combination pharmacokinetic research on Chinese herbal medicines.中药多成分及药物组合的药代动力学研究。
Acta Pharmacol Sin. 2022 Dec;43(12):3080-3095. doi: 10.1038/s41401-022-00983-7. Epub 2022 Sep 16.