Li Cui, Wan Jianqing, Wang Deli, Xiao Lu, Li Xuni, Zhang Cunshuai, Wang Zhao
China Institute of Veterinary Drug Control, Beijing 100081, China.
School of Laboratory Animal & Shandong Laboratory Animal Center, Shandong First Medical University, Jinan 250117, China.
Viruses. 2025 Jan 14;17(1):104. doi: 10.3390/v17010104.
Japanese encephalitis (JE) is a zoonotic disease caused by the Japanese encephalitis virus (JEV), belonging to the family. Diagnosis of Japanese encephalitis (JE) based on clinical signs alone is challenging due to the high proportion of subclinical cases. The Plaque Reduction Neutralization Test (PRNT) is considered the gold standard for detecting JE-specific antibodies because of its high specificity. However, PRNT is complex, time-consuming, and requires live viruses, limiting its applicability in routine diagnostics. In this study, we compared the sensitivity and correlation of the Hemagglutination Inhibition (HI) assay and PRNT for detecting JE antibodies in avian serum samples. We conducted a comparative analysis of the outcomes obtained from the PRNT and HI using 240 serum samples collected from 30 JEV-immunized avian subjects at various time points. Comparative analysis revealed a significant correlation between the HI and PRNT (R = 0.9321, ≤ 0.0001). The Bland-Altman analysis also exhibited favorable concordance between the two assays. Consequently, HI may function as a viable substitute for PRNT in the screening of a substantial number of serum samples.
日本脑炎（JE）是一种由日本脑炎病毒（JEV）引起的人畜共患病，属于黄病毒科。由于亚临床病例比例高，仅基于临床症状诊断日本脑炎（JE）具有挑战性。空斑减少中和试验（PRNT）因其高特异性，被认为是检测JE特异性抗体的金标准。然而，PRNT复杂、耗时且需要活病毒，限制了其在常规诊断中的应用。在本研究中，我们比较了血凝抑制（HI）试验和PRNT在检测禽类血清样本中JE抗体的敏感性和相关性。我们对从30只JEV免疫禽类受试者在不同时间点采集的240份血清样本进行PRNT和HI的结果进行了比较分析。比较分析显示HI和PRNT之间存在显著相关性（R = 0.9321，P≤0.0001）。Bland-Altman分析也显示两种检测方法具有良好的一致性。因此，在大量血清样本的筛查中，HI可作为PRNT的可行替代方法。
