Chen Yuanling, Chen Gaoxiang, Yu Jiaping, Zhou Yali, Fei Shifang, Chen Haorong, Wu Jianxiang, Fu Shuai
Research Center for Life Sciences Computing, Zhejiang Lab, Hangzhou 311100, China.
Hainan Institute, Zhejiang University, Sanya 572025, China.
Viruses. 2025 Jan 15;17(1):114. doi: 10.3390/v17010114.
, a medicinal herbaceous plant documented in the Chinese Pharmacopoeia, is a promising candidate for research into plant-derived pharmaceuticals. However, the study of newly emerging viruses that threaten the cultivation of remains limited. In this study, plants exhibiting symptoms such as leaf yellowing, mottled leaves, and vein chlorosis were collected and subjected to RNA sequencing to identify potential viral pathogens. A novel polerovirus, named Viola Philippica Polerovirus (VPPV), was identified in . VPPV possesses a linear, positive-sense, single-stranded RNA genome consisting of 5535 nucleotides (nt) and encodes seven highly overlapping open reading frames (ORFs). Two potential recombination events were identified within ORF2, ORF3a, and ORF3, providing insights into the genetic diversity and evolution history of this novel polerovirus. An infectious cDNA clone of VPPV was successfully constructed and shown to infect . Using a PVX-based heterologous expression system, the VPPV P0 protein was shown to trigger a systemic hypersensitive response (HR)-like reaction in , indicating that P0 functions as the main pathogenicity determinant. These findings contributed to the detection and understanding of pathogenic mechanisms and control strategies for VPPV in .
作为一种被《中国药典》收录的药用草本植物，是植物源药物研究的一个有前景的候选对象。然而，对威胁其种植的新出现病毒的研究仍然有限。在本研究中，收集了表现出叶片发黄、斑驳叶和叶脉黄化等症状的植物，并对其进行RNA测序以鉴定潜在的病毒病原体。在中鉴定出一种新型的马铃薯卷叶病毒，命名为紫花地丁马铃薯卷叶病毒（VPPV）。VPPV拥有一个由5535个核苷酸（nt）组成的线性、正义、单链RNA基因组，并编码七个高度重叠的开放阅读框（ORF）。在ORF2、ORF3a和ORF3内鉴定出两个潜在的重组事件，为这种新型马铃薯卷叶病毒的遗传多样性和进化历史提供了见解。成功构建了VPPV的感染性cDNA克隆，并证明其能感染。使用基于PVX的异源表达系统，VPPV的P0蛋白在中引发了类似系统性过敏反应（HR）的反应，表明P0作为主要的致病性决定因素发挥作用。这些发现有助于检测和理解VPPV在中的致病机制和控制策略。
