Zhou Benguo, Wang Fang, Zhang Xuesong, Zhang Lina, Lin Huafeng
College of Plant Protection, Anhui Agricultural University, Hefei, 230036, Anhui, China.
Tobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031, Anhui, China.
Arch Virol. 2017 Jul;162(7):2159-2162. doi: 10.1007/s00705-017-3339-8. Epub 2017 Mar 24.
The complete genome sequence of a new virus, provisionally named tobacco virus 2 (TV2), was determined and identified from leaves of tobacco (Nicotiana tabacum) exhibiting leaf mosaic, yellowing, and deformity, in Anhui Province, China. The genome sequence of TV2 comprises 5,979 nucleotides, with 87% nucleotide sequence identity to potato leafroll virus (PLRV). Its genome organization is similar to that of PLRV, containing six open reading frames (ORFs) that potentially encode proteins with putative functions in cell-to-cell movement and suppression of RNA silencing. Phylogenetic analysis of the nucleotide sequence placed TV2 alongside members of the genus Polerovirus in the family Luteoviridae. To the best our knowledge, this study is the first report of a complete genome sequence of a new polerovirus identified in tobacco.
从中国安徽省表现出叶片花叶、黄化和畸形的烟草（Nicotiana tabacum）叶片中，测定并鉴定出一种新病毒的完整基因组序列，该病毒暂命名为烟草病毒2（TV2）。TV2的基因组序列由5979个核苷酸组成，与马铃薯卷叶病毒（PLRV）的核苷酸序列同一性为87%。其基因组结构与PLRV相似，包含六个开放阅读框（ORF），这些开放阅读框可能编码在细胞间移动和RNA沉默抑制中具有推定功能的蛋白质。对核苷酸序列的系统发育分析将TV2与黄症病毒科马铃薯卷叶病毒属的成员归为一类。据我们所知，本研究是在烟草中鉴定出的一种新马铃薯卷叶病毒完整基因组序列的首次报道。
