Mohan Tamilselvan, Bračič Matej, Bračič Doris, Lackner Florian, Nagaraj Chandran, Štiglic Andreja Dobaj, Kargl Rupert, Kleinschek Karin Stana
Graz University of Technology, Institute for Chemistry and Technology of Biobased System (IBioSys), Stremayrgasse 9, 8010 Graz, Austria; University of Maribor, Faculty of Mechanical Engineering, Laboratory for Characterization and Processing of Polymers, Smetanova Ulica 17, 2000 Maribor, Slovenia.
University of Maribor, Faculty of Mechanical Engineering, Laboratory for Characterization and Processing of Polymers, Smetanova Ulica 17, 2000 Maribor, Slovenia.
STAR Protoc. 2025 Mar 21;6(1):103583. doi: 10.1016/j.xpro.2024.103583. Epub 2025 Jan 24.
Three-dimensional (3D) and porous scaffolds made from nanocellulosic materials hold significant potential in tissue engineering (TE). Here, we present a protocol for fabricating self-standing (nano)cellulose-based 3D scaffolds designed for in vitro testing of cells from skin and cartilage tissues. We describe steps for preparation of nanocellulose ink, scaffold formation using 3D printing, and freeze-drying. We then detail post-processing procedures to enhance mechanical properties, stability, and biocompatibility. This protocol offers researchers a framework for developing versatile and sustainable biomaterials for regenerative medicine. For complete details on the use and execution of this protocol, please refer to Mohan et al. and Štiglic et al..
由纳米纤维素材料制成的三维（3D）多孔支架在组织工程（TE）中具有巨大潜力。在此，我们展示了一种制备自立式（纳米）纤维素基3D支架的方案，该支架专为皮肤和软骨组织细胞的体外测试而设计。我们描述了纳米纤维素墨水的制备步骤、使用3D打印形成支架以及冷冻干燥的过程。然后，我们详细介绍了后处理程序，以增强机械性能、稳定性和生物相容性。该方案为研究人员提供了一个开发用于再生医学的多功能且可持续生物材料的框架。有关此方案的使用和执行的完整详细信息，请参考莫汉等人以及斯蒂格利克等人的研究。
