Hamdi Marwa, Wang Dandan, Li Suming, Su Feng
College of Chemistry and Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
College of Chemistry and Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Int J Biol Macromol. 2025 Apr;300:140296. doi: 10.1016/j.ijbiomac.2025.140296. Epub 2025 Jan 23.
A vast sum of fish waste is being annually discarded by marine fishing industries imposing serious environmental pollution concerns. However, these aquatic discarded matters are captivating sources of collagen, a fibrous protein with eminent social and economic relevance. Collagen is conventionally recovered using outdated complex processes requiring many reagents, multiple steps, and extended periods. Hereupon, the current project is the first work on the isolation of Seabass fish scales (FSC) type-I collagen, with preserved secondary and triple helical structures of the native collagen, developing a simple, green, cost-effective, and eco-friendly methodology, utilizing sustainable natural deep eutectic solvents (NADES)-assisted ultrasonication (US) technical route. The operational conditions were optimized based on the one-factor-at-a-time modeling to maximize the yield with no alteration of collagen integrity. Recorded data confirmed type-I collagen with preserved triple helix integrity and thermal stability, improved bio-functionalities, in vitro fibril formation, and functional performances. Finally, the in vitro hemolysis and cytotoxicity tests confirmed the extracted collagens biocompatibility, demonstrating the feasibility of Seabass FSC waste and a NADES-coupled US brief process (20 min) to establish a more sustainable eco-friendly pathway to isolate high-quality type I-collagen, as an attempt to rise industries awareness about wastes valorization within the scheme of circular economy.
海洋捕捞业每年都会丢弃大量的鱼废料，这引发了严重的环境污染问题。然而，这些水生废弃物却是胶原蛋白的诱人来源，胶原蛋白是一种具有重要社会和经济意义的纤维蛋白。传统上，胶原蛋白的提取采用的是过时的复杂工艺，需要许多试剂、多个步骤，且耗时较长。因此，本项目首次开展了从海鲈鱼鱼鳞（FSC）中分离I型胶原蛋白的工作，保留了天然胶原蛋白的二级和三螺旋结构，开发了一种简单、绿色、经济高效且环保的方法，采用可持续的天然低共熔溶剂（NADES）辅助超声（US）技术路线。基于一次单因素建模对操作条件进行了优化，以在不改变胶原蛋白完整性的情况下最大化产量。记录的数据证实了I型胶原蛋白保留了三螺旋完整性和热稳定性，改善了生物功能、体外原纤维形成和功能性能。最后，体外溶血和细胞毒性测试证实了提取的胶原蛋白具有生物相容性，证明了利用海鲈鱼FSC废料和NADES耦合超声的简短工艺（20分钟）来建立一条更可持续的环保途径以分离高质量I型胶原蛋白的可行性，以此提高行业对循环经济框架内废物增值的认识。
