Kon' I Ia, Gorgoshidze L Sh
Biull Eksp Biol Med. 1985 Apr;99(4):428-9.
It has been shown in experiments in vitro that preincubation of rat liver microsomes with an ethanol solution of all-trans-retinoic acid in the final concentration 7.0 X 10(-5) M results in a decrease of both NADPN-dependent and spontaneous lipid peroxidation (to 53 and 70% of control, respectively) but did not influence ascorbate-dependent lipid peroxidation. Retinol at the same concentration induces more pronounced inhibition of all types of microsomal lipid peroxidation. The rate of NADPN-dependent lipid peroxidation decreases linearly as the retinoic acid concentration in the incubation medium is raised, whereas the rate of ascorbate-dependent lipid peroxidation drastically lessens only after the retinoic acid concentration in the medium is increased to 1.4 X 10(-4) M. The data obtained provide evidence in favour of the concepts of a possible role of vitamin A in LPO regulation in the body and point to the necessity of taking into consideration the antioxidant properties of retinol and retinoic acid while analysing their pharmacological action.
体外实验表明，将大鼠肝脏微粒体与终浓度为7.0×10⁻⁵ M的全反式视黄酸乙醇溶液预孵育，会导致NADPN依赖性和自发性脂质过氧化作用均降低（分别降至对照的53%和70%），但不影响抗坏血酸依赖性脂质过氧化作用。相同浓度的视黄醇对所有类型的微粒体脂质过氧化作用具有更显著的抑制作用。随着孵育介质中视黄酸浓度的升高，NADPN依赖性脂质过氧化作用的速率呈线性下降，而只有当介质中视黄酸浓度增加到1.4×10⁻⁴ M后，抗坏血酸依赖性脂质过氧化作用的速率才会急剧降低。所获得的数据支持了维生素A在体内脂质过氧化作用调节中可能发挥作用的观点，并指出在分析视黄醇和视黄酸的药理作用时，有必要考虑它们的抗氧化特性。
