Zhang Jinyu, Zou Liying, Wang Li, Zhang Dongchao, Shen Ao, Lei Yongqi, Chao Maoni, Xu Xinjuan, Xue Zhiwei, Huang Zhongwen
Henan Collaborative Innovation Center of Modern Biological Breeding, College of Agronomy, Henan Institute of Science and Technology, Xinxiang, 453003, China.
Anyang Academy of Agricultural Sciences, Anyang, 455000, China.
BMC Genomics. 2025 Jan 25;26(1):73. doi: 10.1186/s12864-025-11270-0.
The Sec14 domain is an ancient lipid-binding domain that evolved from yeast Sec14p and performs complex lipid-mediated regulatory functions in subcellular organelles and intracellular traffic. The Sec14 family is characterized by a highly conserved Sec14 domain, and is ubiquitously expressed in all eukaryotic cells and has diverse functions. However, the number and characteristics of Sec14 homologous genes in soybean, as well as their potential roles, remain understudied.
In this study, we identified 77 Sec14 genes in the soybean genome that were unevenly distributed across 19 chromosomes. Based on the classification method used for Arabidopsis Sec14 members, GmSec14s can be categorized into three classes: GmPITP1 to GmPITP37, GmSFH1 to GmSFH25, and GmPATL1 to GmPATL15. Structural analysis of the GmSec14 genes revealed that the SFH subfamily contained more introns than the other subfamilies. A total of 10 conserved protein motifs were detected within GmSec14 proteins, with each subfamily possessing unique motifs. Two tandem duplications and 73 segmental duplications were identified among the GmSec14 genes. Additionally, a large number of cis-acting elements, particularly those related to plant hormones, were abundant in the promoter regions of the GmSec14 genes. Tissue expression analysis of the GmSec14 genes indicated that they exhibited distinct tissue-specific expression patterns. In response to salt stress, multiple genes were found to be either upregulated or downregulated. In contrast, the majority of genes were downregulated under drought stress conditions. Notably, 12 GmSec14 genes exhibited significant alterations in expression following salt or drought stress, suggesting a potential role for these genes in stress response mechanisms. Furthermore, the protein interaction network and miRNA regulation associated with GmSec14s were predicted to elucidate the potential functions of GmSec14 members.
This study provides a systematic and comprehensive examination of the Sec14 gene family in soybean, which will facilitate further functional research into their roles in response to salt and drought tolerance.
Sec14结构域是一个古老的脂质结合结构域，由酵母Sec14p进化而来，在亚细胞器和细胞内运输中执行复杂的脂质介导的调节功能。Sec14家族的特征是具有高度保守的Sec14结构域，在所有真核细胞中普遍表达且具有多种功能。然而，大豆中Sec14同源基因的数量、特征及其潜在作用仍未得到充分研究。
在本研究中，我们在大豆基因组中鉴定出77个Sec14基因，它们不均匀地分布在19条染色体上。根据用于拟南芥Sec14成员的分类方法，GmSec14s可分为三类：GmPITP1至GmPITP37、GmSFH1至GmSFH25以及GmPATL1至GmPATL15。对GmSec14基因的结构分析表明，SFH亚家族比其他亚家族含有更多的内含子。在GmSec14蛋白中总共检测到10个保守的蛋白质基序，每个亚家族都有独特的基序。在GmSec14基因中鉴定出两个串联重复和73个片段重复。此外，GmSec14基因的启动子区域富含大量顺式作用元件，特别是那些与植物激素相关的元件。GmSec14基因的组织表达分析表明它们表现出明显的组织特异性表达模式。在盐胁迫下，发现多个基因上调或下调。相比之下，在干旱胁迫条件下，大多数基因下调。值得注意的是，12个GmSec14基因在盐或干旱胁迫后表达发生显著变化，表明这些基因在胁迫响应机制中可能发挥作用。此外，预测了与GmSec14s相关的蛋白质相互作用网络和miRNA调控，以阐明GmSec14成员的潜在功能。
本研究对大豆中的Sec14基因家族进行了系统而全面的研究，这将有助于进一步研究它们在盐和干旱耐受性响应中的作用。
