Abe S, Kumanishi T, Araki S, Satake M
Brain Res. 1985 Feb 18;327(1-2):259-67. doi: 10.1016/0006-8993(85)91519-7.
A group of novel phosphonoglycosphingolipids was isolated from the tissues of Aplysia. In the present experiment, antiserum was raised against total phosphonoglycosphingolipids isolated from the ganglia. This antiserum seems specific to the oligosaccharide moiety of the glycolipids. It did not react with gangliosides isolated from mammalian brain. Of the total phosphonoglycosphingolipids of the ganglion. GGL-V was strongly reactive, but GGL-I was hardly reactive with the antiserum. The indirect immunoperoxidase method in combination with light microscopy revealed staining of fibrous structures in the neuropil of ganglia, connective tracts and peripheral nerves. These fibrous structures often interconnected with supporting cells (glia cells). However, the neuron and its processes were stained not distinctly. Thus our results indicate that some of the major glycolipids isolated from the ganglion are mainly present in extraneuronal components in the nervous tissues of Aplysia.
从海兔组织中分离出了一组新型的膦酸糖鞘脂。在本实验中，制备了针对从神经节分离出的总膦酸糖鞘脂的抗血清。这种抗血清似乎对糖脂的寡糖部分具有特异性。它与从哺乳动物脑部分离出的神经节苷脂不发生反应。在神经节的总膦酸糖鞘脂中，GGL-V具有强烈的反应性，但GGL-I与抗血清几乎没有反应性。间接免疫过氧化物酶法结合光学显微镜显示，神经节神经毡、结缔组织束和外周神经中的纤维结构被染色。这些纤维结构常常与支持细胞（神经胶质细胞）相互连接。然而，神经元及其突起的染色并不明显。因此，我们的结果表明，从神经节分离出的一些主要糖脂主要存在于海兔神经组织的神经外成分中。
