外周经典单核细胞亚群和中间单核细胞亚群作为系统性红斑狼疮疾病活动的免疫生物标志物

Peripheral Classic and Intermediate Monocyte Subsets as Immune Biomarkers of Systemic Lupus Erythematosus Disease Activity.

作者信息

Oehadian Amaylia, Ghozali Mohammad, Kusuma Sutiadi, Mersiana Lusi, Ghassani Nadia Gita, Fransisca Fransisca, Sigilipu Yitzchak Millenard, Kartikasari Andini, Hamijoyo Laniyati

机构信息

Hematology Division, Department of Internal Medicine, Faculty of Medicine, Padjadjaran University - Hasan Sadikin Hospital, Bandung, Indonesia.

出版信息

Acta Med Indones. 2024 Oct;56(4):469-475.

PMID:39865061

Abstract

BACKGROUND

Monocytes are evolutionarily preserved innate immune cells that play essential roles in immune response regulation. Three activated monocyte subsets-classical (CD14++CD16-), intermediate (CD14++CD16+), and nonclassical (CD14+CD16++)-are associated with systemic lupus erythematosus (SLE) progression. This study aims to determine the association of monocyte subsets with SLE disease activity.

METHODS

A cross-sectional study involving 25 patients with SLE was conducted. Blood samples were collected, and monocyte subsets were identified using flow cytometry. Patients were grouped by disease activity using the Systemic Lupus Erythematosus Disease Activity Index 2000 (SLEDAI-2K) into inactive (SLEDAI-2K ≤ 4) and active (SLEDAI-2K > 4). The cutoff for monocyte subsets was determined using Receiver Operating Characteristic (ROC) analysis.

RESULTS

Nine active and 16 inactive subjects were identified. Compared with individuals without active disease, individuals with active disease had significantly lower mean classical monocyte subsets (71.9% vs 88%, p = 0.008), and higher median intermediate monocytes (29.1% vs 11.1%, p = 0.019). The median nonclassical monocyte subsets were not significantly different between the two groups. The cutoff for classical monocytes in active disease was ≤72.2%, AUC = 0.788, p = 0.021, with 66.7% sensitivity and 87.5% specificity; for intermediate monocytes, it was >22.3%, AUC = 0.788, p = 0.014, with 66.7% sensitivity and 100% specificity.

CONCLUSION

Classical and intermediate monocytes could be considered as immune cellular markers for identifying active SLE.

摘要

背景

单核细胞是进化上保守的固有免疫细胞，在免疫反应调节中起重要作用。三种活化的单核细胞亚群——经典型（CD14++CD16-）、中间型（CD14++CD16+）和非经典型（CD14+CD16++）——与系统性红斑狼疮（SLE）的进展相关。本研究旨在确定单核细胞亚群与SLE疾病活动度之间的关联。

方法

对25例SLE患者进行了一项横断面研究。采集血样，并用流式细胞术鉴定单核细胞亚群。使用2000年系统性红斑狼疮疾病活动指数（SLEDAI-2K）将患者按疾病活动度分为非活动组（SLEDAI-2K≤4）和活动组（SLEDAI-2K>4）。使用受试者工作特征（ROC）分析确定单核细胞亚群的临界值。

结果

确定了9例活动期和16例非活动期受试者。与无活动性疾病的个体相比，活动性疾病个体的平均经典单核细胞亚群显著降低（71.9%对88%，p=0.008），中间单核细胞的中位数较高（29.1%对11.仁，p=0.019）。两组之间非经典单核细胞亚群的中位数无显著差异。活动期疾病中经典单核细胞的临界值≤72.2%，AUC=0.788，p=0.021，敏感性为66.7%，特异性为87.5%；中间单核细胞的临界值>22.3%，AUC=0.788，p=0.014，敏感性为66.7%，特异性为100%。