Ito Ryoma, Komaki Yukako, Ibuki Yuko
Graduate Division of Nutritional and Environmental Sciences, University of Shizuoka, Yada 52- 1, Suruga-ku, Shizuoka, 422-8526, Japan.
Genes Environ. 2025 Jan 26;47(1):2. doi: 10.1186/s41021-025-00325-z.
Skin is exposed to various environmental factors throughout life, and some of these factors are known to contribute to skin aging. Long-term solar UV exposure is a well-known cause of skin aging, as is cigarette smoke, which contains a number of chemicals. In this study, combined effect of UVA and cigarette sidestream smoke (CSS) on matrix metalloproteinase-1 (MMP-1) induction was investigated. MMP-1 is the main protease that initiates collagen type I fiber fragmentation in human skin and is associated with aging.
Combined exposure to UVA and CSS enhanced MMP-1 induction, accompanied by collagen type I (COL1A1) gene suppression. The basal expression of MMP-1 was higher in senescent cells than in normal cells, with a pronounced increase after coexposure to UVA and CSS. UVA irradiation resulted in global histone H3 acetylation, and we considered this was responsible for the MMP-1 upregulation. Histone deacetylase inhibitors, sodium acetate, propionate, and butyrate, all enhanced the CSS-induced MMP-1 according to the degree of histone acetylation.
These results suggest that UVA and CSS additively induce MMP-1, which may lead to skin aging, and that such combined effect may further promote aging in aged skin. UVA-induced histone acetylation may contribute to MMP-1 induction.
皮肤在其整个生命周期中都会接触到各种环境因素，其中一些因素已知会导致皮肤老化。长期暴露于太阳紫外线是众所周知的皮肤老化原因，香烟烟雾也是如此，它含有多种化学物质。在本研究中，我们调查了紫外线A（UVA）和香烟侧流烟雾（CSS）对基质金属蛋白酶-1（MMP-1）诱导的联合作用。MMP-1是启动人类皮肤中I型胶原纤维断裂的主要蛋白酶，与皮肤老化相关。
UVA和CSS联合暴露增强了MMP-1的诱导，同时伴有I型胶原（COL1A1）基因抑制。MMP-1的基础表达在衰老细胞中高于正常细胞，在UVA和CSS共同暴露后显著增加。UVA照射导致整体组蛋白H3乙酰化，我们认为这是MMP-1上调的原因。组蛋白脱乙酰酶抑制剂，醋酸钠、丙酸钠和丁酸钠，均根据组蛋白乙酰化程度增强了CSS诱导的MMP-1。
这些结果表明，UVA和CSS可叠加诱导MMP-1，这可能导致皮肤老化，并且这种联合作用可能会进一步促进老年皮肤的老化。UVA诱导的组蛋白乙酰化可能有助于MMP-1的诱导。
