Koompapong Khuanchai, Popruk Supaluk, Reamtong Onrapak, Thiangtrongjit Tipparat, Ampawong Sumate, Sakulpanich Aurapa, Udonsom Ruenruetai, Thima Kanthinich, Sreepian Preeyaporn M, Poovorawan Kittiyod, Mori Hirotake, Stensvold Christen Rune, Mahittikorn Aongart
Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Ratchawithi Road, Ratchathewi, Bangkok, 10400, Thailand.
Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Ratchawithi Road, Ratchathewi, Bangkok, 10400, Thailand.
Heliyon. 2025 Jan 2;11(1):e41530. doi: 10.1016/j.heliyon.2024.e41530. eCollection 2025 Jan 15.
is a major pathogen of the gastrointestinal tract, and infections impact both human health and the economy. The ongoing issues with drug resistance and the side effects of current anti- treatments highlight the urgent need for new therapeutic options. This study focused on investigating the efficacy of crude extracts of from Thailand against . was initially extracted with hexane, followed sequentially by dichloromethane, ethanol, and water. The extracts were tested for growth inhibition against . Dichloromethane and hexane extracts were highly active against the parasite after 48 h of exposure, with half-maximal inhibitory concentration (IC50) values of 60.77 μg/mL and 66.66 μg/mL, respectively. Both extracts reduced trophozoite motility and adherence, altered trophozoite architecture, and induced apoptosis. Our findings demonstrate the anti- activity of root extracts, especially those of dichloromethane and hexane. Our results will support the potential development of new and effective medications for the treatment of giardiasis.
是胃肠道的主要病原体，感染会影响人类健康和经济。目前耐药性问题以及现有抗治疗药物的副作用凸显了对新治疗选择的迫切需求。本研究聚焦于调查泰国的粗提物对的疗效。最初用己烷提取，随后依次用二氯甲烷、乙醇和水提取。测试提取物对的生长抑制作用。二氯甲烷和己烷提取物在暴露48小时后对该寄生虫具有高活性，半数最大抑制浓度（IC50）值分别为60.77μg/mL和66.66μg/mL。两种提取物均降低了滋养体的运动性和黏附性，改变了滋养体结构，并诱导了细胞凋亡。我们的研究结果证明了根提取物的抗活性，尤其是二氯甲烷和己烷提取物。我们的结果将支持开发用于治疗贾第虫病的新型有效药物的潜力。
