健康个体的血清中可反映出口服补充丙酸盐的情况。
Oral supplementation with propionate is reflected in the serum of healthy individuals.
作者信息
Schröder Maximilian, Pasic Arijan, Hirche Frank, Rozanova Svitlana, Sgodzai Melissa, Gisevius Barbara, Horstkemper Lea, Gold Ralf, Marcus Katrin, Pitarokoili Kalliopi, Motte Jeremias, Barkovits Katalin, Stangl Gabriele, Fisse Anna Lena
机构信息
Department of Neurology, St. Josef-Hospital, Ruhr-University Bochum, Germany.
Medizinisches Proteom-Center, Medical Faculty, Ruhr-University Bochum, Germany.
出版信息
Ther Adv Neurol Disord. 2025 Jan 23;18:17562864241309755. doi: 10.1177/17562864241309755. eCollection 2025.
BACKGROUND
Short-chain fatty acids (SCFAs), including propionic acid (PA), are key in immunological research. Supplementing PA has shown benefits for autoimmune diseases. A comprehensive understanding of the PA pharmacokinetics is essential for the optimal design and execution of studies utilizing orally administered PA.
OBJECTIVE
We propose two methods of measuring PA in serum, carried out by different laboratories.
DESIGN
Blood samples from 20 volunteers were collected hourly following PA supplementation.
METHODS
Serum propionate quantification was performed with two independent mass spectrometry-based (MS) analyses, including liquid-chromatography (LC)-MS and direct-infusion (DI)-MS.
RESULTS
PA levels increased within 1 h of ingestion of 500 mg PA. Serum concentrations ranged from 1.3 to 4.5 µmol/L, rising significantly after 1 h ( < 0.05). Serum levels returned to baseline within 2 h. No significant differences were found regarding sex or diet.
CONCLUSION
The shown pharmacokinetics can be used in future PA research.
背景
包括丙酸（PA）在内的短链脂肪酸是免疫学研究的关键。补充PA已显示出对自身免疫性疾病有益。全面了解PA的药代动力学对于利用口服PA的研究的最佳设计和实施至关重要。
目的
我们提出了两种由不同实验室进行的血清中PA测量方法。
设计
在补充PA后每小时收集20名志愿者的血样。
方法
采用两种基于质谱（MS）的独立分析方法进行血清丙酸盐定量，包括液相色谱（LC）-MS和直接进样（DI）-MS。
结果
摄入500mg PA后1小时内PA水平升高。血清浓度范围为1.3至4.5µmol/L，1小时后显著升高（<0.05）。血清水平在2小时内恢复到基线。在性别或饮食方面未发现显著差异。
结论
所示的药代动力学可用于未来的PA研究。
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