Ali Hussein M, Attia Mohamed H, Ramadan Khaled M A, Rashed Eman N, Bendary Eslam S
Agricultural Biochemistry Department, Faculty of Agriculture, Ain-Shams University, P.O. Box 68, Hadayek Shoubra, Cairo 11241 Egypt.
Central Laboratories, King Faisal University, 31982 Al-Ahsa, Saudi Arabia.
J Food Sci Technol. 2025 Jan;62(1):75-87. doi: 10.1007/s13197-024-06011-2. Epub 2024 Jul 23.
Despite the remarkably high antioxidant activity of tocopherols, their applications in the food industry are limited because of their instability under various conditions. Complexes of α-tocopherol (α-TQ) or α-tocopheryl acetate (α-TQA) with β-cyclodextrin (β-CD) or starch were prepared and characterized by UV-vis, IR and thermal analysis. Oxidative stability of α-TQ and α-TQA against HO was 74.7 and 88.8% respectively (576 h). However, stability increased to 82.9 and 100% for β-CD and 99.2 and 99.4% for starch complexes respectively, which indicates that starch is an excellent stabilizing host in addition to being an economic material. Stability of α-TQ and α-TQA under light conditions was dependent on their physical state; it was 55.3 and 82.9% (oil) but stability was lowered to 19.4 and 76.5% in solution (2.21 mM). In addition, exposure to UV irradiation decreased their stability to 39.2 and 85.0% as oil and 61.2 and 89.1% in solution respectively. However, the stability of all complexes remained > 99.0% under light and UV conditions. Accordingly, α-TQ, the natural and biologically active form, can be used as complex rather than α-TQA often used because of its higher stability. Docking revealed that both forms fit in β-CD with the side chain taking "U" conformation.
The online version contains supplementary material available at 10.1007/s13197-024-06011-2.
尽管生育酚具有极高的抗氧化活性，但由于其在各种条件下的不稳定性，它们在食品工业中的应用受到限制。制备了α-生育三烯酚（α-TQ）或α-生育酚乙酸酯（α-TQA）与β-环糊精（β-CD）或淀粉的复合物，并通过紫外可见光谱、红外光谱和热分析对其进行了表征。α-TQ和α-TQA对羟基自由基（HO）的氧化稳定性分别为74.7%和88.8%（576小时）。然而，β-CD复合物的稳定性分别提高到82.9%和100%，淀粉复合物的稳定性分别提高到99.2%和99.4%，这表明淀粉除了是一种经济的材料外，还是一种优异的稳定主体。α-TQ和α-TQA在光照条件下的稳定性取决于它们的物理状态；在油相中分别为55.3%和82.9%，但在溶液中（2.21 mM）稳定性降至19.4%和76.5%。此外，暴露于紫外线照射下，其在油相中的稳定性分别降至39.2%和85.0%，在溶液中分别降至61.2%和89.1%。然而，所有复合物在光照和紫外线条件下的稳定性均保持>99.0%。因此，天然且具有生物活性的α-TQ形式可以作为复合物使用，而不是经常使用的α-TQA，因为其具有更高的稳定性。对接结果显示，两种形式都以侧链呈“U”构象的方式适配于β-CD。
在线版本包含可在10.1007/s13197-024-06011-2获取的补充材料。
