Pollegioni Loredano, Molla Gianluca
'The Protein Factory 2.0', Dipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell'Insubria, Varese, Italy.
FEBS J. 2025 May;292(10):2511-2514. doi: 10.1111/febs.17416. Epub 2025 Jan 27.
The sequestration of carbon dioxide using carbonic anhydrase (CA) is one of the most effective methods for mitigating global warming. The burning of fossil fuels releases large quantities of flue gas; because of its high temperature and of the alkaline conditions required for CaCO precipitation in the mineralization process, thermo-alkali-stable CAs are needed. In this context, Manyumwa et al. conducted a biochemical characterization of three CAs derived from thermophilic bacteria. They then employed a rational design approach to enhance the specific activity and stability of the enzyme from the hydrothermal vent species Persephonella sp. KM09-Lau-8.
利用碳酸酐酶（CA）封存二氧化碳是缓解全球变暖最有效的方法之一。化石燃料燃烧会释放大量烟道气；由于其温度高且矿化过程中碳酸钙沉淀需要碱性条件，因此需要热碱稳定的碳酸酐酶。在此背景下，曼尤姆瓦等人对三种嗜热细菌来源的碳酸酐酶进行了生化特性分析。然后，他们采用合理设计方法提高了来自热液喷口物种佩尔塞福涅菌属KM09-Lau-8的酶的比活性和稳定性。
