Thorud E, Aarnaes E, Clausen O P, Bjerknes R, Anker P
Cell Tissue Kinet. 1985 May;18(3):279-95. doi: 10.1111/j.1365-2184.1985.tb00658.x.
Circadian variations in the proliferative activity of squamous epithelia are well known. However, circadian variations in the duration of the various cell cycle phases (S, G2 and mitosis) have been disputed. The percent labelled mitoses method, which is traditionally used to obtain duration of cell cycle phases, is poorly suited for identification of circadian variations. Therefore methods combining changes in compartment size (cell cycle phase) and cellular flux through the compartments have been used. Three different methods using such data are presented. These incorporate various simplifying assumptions that cause methodological errors. Limits for use of the different methods are indicated. The use of all three methods gives comparable and pronounced circadian variations in the duration of S and G2 phase. These results are also compatible with circadian variations in the mitotic duration, but they may also represent artefacts due to sensitivity to model errors.
鳞状上皮细胞增殖活性的昼夜节律变化是众所周知的。然而，细胞周期各阶段（S期、G2期和有丝分裂期）持续时间的昼夜节律变化一直存在争议。传统上用于获取细胞周期各阶段持续时间的标记有丝分裂百分数法，不太适合识别昼夜节律变化。因此，已采用结合区室大小（细胞周期阶段）变化和通过区室的细胞通量的方法。介绍了三种使用此类数据的不同方法。这些方法包含各种导致方法误差的简化假设。指出了不同方法的使用限制。使用这三种方法均得出S期和G2期持续时间具有可比性且明显的昼夜节律变化。这些结果也与有丝分裂持续时间的昼夜节律变化相符，但它们也可能是由于对模型误差敏感而产生的假象。
