Potten C S, Al-Barwari S E, Hume W J, Searle J
Cell Tissue Kinet. 1977 Nov;10(6):557-68. doi: 10.1111/j.1365-2184.1977.tb00312.x.
Variation in the percentage of labelled cells (LI), mitoses (MI) and apoptosis (AI: i.e. shrinkage necrosis) have been studied throughout a 24 hr period (40 min after labelling with 3H-TdR) for tongue epithelium, epidermis and intestinal epithelium in the mouse. A room with reversed light cycle was used to obtain data for half of the 24 hr period. All three tissues showed marked variations in LI with peak values between 24.00 and 03.00 hours. In the intestine a maximum value for MI was observed 3-6 hr after that for LI and with a maximum value for AI slightly later. In all three epithelia the circadian rhythm was most striking in cells at positions which can be correlated with presumptive stem cell activity; e.g. in the crypts the labelling and mitotic peaks reflecting a circadian rhythm were most clearly distinguishable at the basal part of the crypts. These observations are discussed in relation to the validity of various proliferative models.
在小鼠的舌上皮、表皮和肠上皮中，研究了标记细胞百分比（LI）、有丝分裂（MI）和细胞凋亡（AI，即收缩性坏死）在整个24小时期间（用³H-TdR标记后40分钟）的变化情况。使用了一个光照周期颠倒的房间来获取24小时周期中一半时间的数据。所有这三种组织的LI均表现出明显变化，峰值出现在24:00至03:00之间。在肠道中，MI的最大值在LI最大值出现后3 - 6小时观察到，AI的最大值稍晚出现。在所有这三种上皮组织中，昼夜节律在与假定干细胞活性相关的位置的细胞中最为明显；例如，在隐窝中，反映昼夜节律的标记和有丝分裂峰值在隐窝底部最清晰可辨。结合各种增殖模型的有效性对这些观察结果进行了讨论。
